Barley (Hordeum vulgare subsp. vulgare) 2n = 2x = 14 ~5,000 Mb Long history of genetics research Economically important crop Malting/brewing, food, feed, forage Model for polyploid Triticeae

Oregon Wolfe Barley Mapping Population Doubled haploid International open resource Morphological, isozyme, RFLP, SSR, AFLP, DArT, SNP data Linkage maps QTLs

Considerations in mapping: a RAD case study

Why the OWB? Maximum polymorphism and anchor markers

RAD maps compared to prior maps # of linkage groups ✔ Order ✔ Distance ✔ Gap filling ~

RAD maps compared to prior maps Quality ✔

RAD maps and compatibility Integration with prior data ✔

RAD maps and applications Utility ✔

RAD maps and applications Synteny ✔

Uses of linkage maps and QTLs Marker Assisted Selection Genome Wide Association Scans Genomic Selection Isolation and/manipulation of candidate genes

Ideal markers for molecular plant breeding Codominant Consistent genome coordinates across accessions Discriminate between gene family members Gene-based (and/or) based on functional determinants of phenotype Integrated with prior data

Ideal genotyping service for plant breeding DNA extraction included – seed chip ideal Quick turnaround Cost

Linkage maps – souvenir horseshoes or workhorses? Breeders make crosses Actual recombination is critical Detect chromosome rearrangements

Acknowledgements: Generations of OWB practitioners OWB-dominant x OWB recessive F1 Doubled haploids https://barleyworld.org/owb