Single nucleotide polymorphism-based molecular typing of M

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Presentation transcript:

Single nucleotide polymorphism-based molecular typing of M Single nucleotide polymorphism-based molecular typing of M. leprae from multicase families of leprosy patients and their surroundings to understand the transmission of leprosy R.P. Turankar, M. Lavania, V.S. Chaitanya, U. Sengupta, J. Darlong, F. Darlong, K.S.R. Siva Sai, R.S. Jadhav Clinical Microbiology and Infection Volume 20, Issue 3, Pages O142-O149 (March 2014) DOI: 10.1111/1469-0691.12365 Copyright © 2014 European Society of Clinical Infectious Diseases Terms and Conditions

Fig. 1 Detection of Mycobacterium leprae from environmental samples targeting the RLEP region. PCR amplification of the RLEP region of M. leprae obtained from the environmental samples from Purulia. PCR product was electrophoresed on a 2% agarose gel. Samples were: lane 1, negative control; lane 2, positive control; lane 3, 100 bp ladder, lanes 4–11, environmental samples. Clinical Microbiology and Infection 2014 20, O142-O149DOI: (10.1111/1469-0691.12365) Copyright © 2014 European Society of Clinical Infectious Diseases Terms and Conditions

Fig. 2 Polymerase chain reaction restriction fragment length polymorphism of locus-2. Product was electrophoresed on 4% agarose gel. Samples were: lanes 1, 3, 5, 7, unrestricted sample; restricted 2, 4, 6, 8 and lane 9, Thai strain unrestricted sample; lane 10 restricted, Thai strain. Clinical Microbiology and Infection 2014 20, O142-O149DOI: (10.1111/1469-0691.12365) Copyright © 2014 European Society of Clinical Infectious Diseases Terms and Conditions

Fig. 3 SNP subtype D from slit-skin smears. Clinical Microbiology and Infection 2014 20, O142-O149DOI: (10.1111/1469-0691.12365) Copyright © 2014 European Society of Clinical Infectious Diseases Terms and Conditions

Fig. 4 SNP subtype C from slit-skin smears. Clinical Microbiology and Infection 2014 20, O142-O149DOI: (10.1111/1469-0691.12365) Copyright © 2014 European Society of Clinical Infectious Diseases Terms and Conditions