SEE LOWER CONCENTRATIONS WITH THE NQAD DETECTOR Application note # QAN-0087 ™ APPLICATION NOTES CHROMATOGRAMS PARAMETERS 4.9 mV NQAD GAS: Air FILTER: 1.3 sec GAIN: 1X EVAPORATION TEMP: 35C ELSD GAS: Nitrogen, 1.5 L/min GAIN: 1 EVAPORATION TEMP: 40C HPLC MOBILE PHASE: 25:75 ACN:Water with 0.2% TFA NQAD, 16 ng S/N = 167 ELSD, 16 ng S/N < 3 FLOW RATE: 1.00 mL/min COLUMN: Phenomenex Kinetex C18, 150 x 4.6, 2.6 u COLUMN TEMP: Ambient INJECTION VOLUME :1 µL CONCENTRATION: 16 ng on column ANALYTE: Ephedrine Demonstrates better sensitivity than ELSD 4.3 0.00 1.00 2.00 3.0 QUANT TECHNOLOGIES, LLC 1463 94TH Lane NE Blaine, MN, USA 55449 763-398-0508 www.Quant-NQAD.com Note: This application was run at Quant Technologies on a single ELSD and may not be representative of all ELSD’s. 1