THE NQAD IS HIGHLY SENSITIVE, ALLOWING MORE SAMPLE COMPONENTS TO BE DETECTED Application note # QAN-0058 CHROMATOGRAMS FLOW RATE: 1.0 mL/min COLUMN: Thermo BetaBasic C18, 150 x 4.6 mm, 5u COLUMN TEMP: Ambient INJECTION VOLUME :10 µL ANALYTE: Hydrocortisone NQAD GAS: Air FILTER: 1.3 sec GAIN: 1X EVAPORATION TEMP: 35C ELSD GAS: Nitrogen, 1.5 L/min GAIN: 1 EVAPORATION TEMP: 40C HPLC MOBILE PHASE: 45:55 water:ACN PARAMETERS 0.00 0.20 0.40 0.60 0.80 1.00 1.20 1.40 1.60 1.80 2.00 2.20 2.40 2.60 2.80 3.00 4.00 9.00 mV min 1 NQAD, 10 ng Avg S/N, 53.5 APPLICATION NOTES 2 0.00 0.20 0.40 0.60 0.80 1.00 1.20 1.40 1.60 1.80 2.00 2.20 2.40 2.60 2.80 3.00 45.00 48.00 mV min 1 ELSD, 10 ng Avg S/N, 2.8 Demonstrates better sensitivity than ELSD ™ QUANT TECHNOLOGIES, LLC 1463 94TH Lane NE Blaine, MN, USA 55449 763-398-0508 www.Quant-NQAD.com 2 1 = Injection Artifact, 2 = Hydrocortisone, 10 ng 1