Supplemental Figure 2 1000 2000 3000 untreated Rituximab Alemtuzumab

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Supplemental Figure 2 1000 2000 3000 untreated Rituximab Alemtuzumab mAb37.1 mAb37.2 lymphocytes / µl B cells T cells Supplemental Fig. 2: Effects of antibody treatment on absolute B and T cell counts in a blood sample from a healthy donor. After 24 h incubation with or without 10 µg/ml of the indicated antibodies, absolute densities of B and T cells in a typical whole blood sample from a healthy donor were determined in duplicate with BD TruCount beads for calibration. In six investigated samples T cell counts were consistently maintained after incubation with the CD37 antibodies and rituximab. B cell depletion by 10 µg/ml of mAb 37.1 in the six samples ranged from 35 to 80 %.