Lab.2: Algal culture media.

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Presentation transcript:

Lab.2: Algal culture media

In their natural habitats algae obtain all the nutrients, minerals and vitamins they require from the water in which they live. To grow them in the lab however, you must provide them with all of these essential resources i.e. you will need to make up some growth media. Algae require many different nutrients in minute quantities that are difficult to anticipate and prepare in the lab. These organisms have different nutritional requirements, thus various kinds of culture media have been developed. Algal media refers to the solution or culture in which algae grow. All the media have several components in common: sources of nitrogen (in the form of nitrate, nitrite and ammonia), phosphorus, vitamins and trace metals. However, the specific types of these nutrients, their concentrations and ratios vary between the media. There are many types of algal culturing media,

the most common algal media are : A-marine algae F/2 Medium: There are many recipes for such algal growth media, F/2 medium however, is the most common and widely used general enriched seawater medium designed for growing marine algae used in the phycological and aquaculture studies.

B-fresh water algae 1-Allen's medium Allen's medium is used for the enhancement of algal growth especially blue-green algae. 2- Chu’s Medium No. 10)Chu-10 medium( Chu-10 is used for the enhancement of most algal species growth.

3-BG-11medium This medium is used for the enhancement of algal growth especially blue-green algae. 4- BG-11medium with minerals This medium is used for the enhancement of algal growth especially blue-green algae

The component function K2HPO4 source of phosphate, it may be responsible for the rapid growth of the algae, this element is comprising higher percentages in the cellular composition as nucleic acid and cellular membrane. Sodium nitrate (NaNO3) Nitrogen being important constituent of the cell protein was needed for algal growth, either in combined or in molecular form. Also constitute basic part with in nucleic acids. Sodium Silicate Silica source, this element is important for group of algae (the diatoms). because they construct their cell wall from silica MgSO4 source of magnesium • magnesium permitted the maximum growth of the present alga and magnesium deficiency interrupted cell division which results in abnormally large cell formation • cells need magnesium to synthesize chlorophyll