Accurate human papillomavirus genotyping by 454 pyrosequencing

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Presentation transcript:

Accurate human papillomavirus genotyping by 454 pyrosequencing V. Militello, E. Lavezzo, G. Costanzi, E. Franchin, B. Di Camillo, S. Toppo, G. Palù, L. Barzon Clinical Microbiology and Infection Volume 19, Issue 10, Pages E428-E434 (October 2013) DOI: 10.1111/1469-0691.12219 Copyright © 2013 European Society of Clinical Infectious Diseases Terms and Conditions

Fig. 1 Results of 454 pyrosequencing analysis of pools of DNA purified from CaSki (HPV16-positive) and HeLa (HPV18-positive) cells. The analysis was performed in duplicate pool samples with PCR primers containing either the multiplex identified MID1 or MID2. Pools contained different proportions of DNA purified from cell lines (and corresponding HPV genome equivalents, GE) ranging from 0.1% (i.e. 1000:1 HPV16 to HPV18) to 80% (i.e. 5:1) HPV16 GE of the total amount of HPV16 and HPV18 GE in samples. Results are represented as absolute number (a) and percentage (b) of reads of HPV18 and HPV16 obtained in samples amplified in duplicate with MID1 and MID2 barcoded primers. Clinical Microbiology and Infection 2013 19, E428-E434DOI: (10.1111/1469-0691.12219) Copyright © 2013 European Society of Clinical Infectious Diseases Terms and Conditions