Protective effect of magnesium on the endothelial function mediated by endothelium- derived hyperpolarizing factor in coronary arteries during cardioplegic.

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Presentation transcript:

Protective effect of magnesium on the endothelial function mediated by endothelium- derived hyperpolarizing factor in coronary arteries during cardioplegic arrest in a porcine model Qin Yang, MD, MPH, Yao-Chung Liu, MD, Wei Zou, MD, Anthony P.C. Yim, MD, Guo-Wei He, MD, PhD The Journal of Thoracic and Cardiovascular Surgery Volume 124, Issue 2, Pages 361-370 (August 2002) DOI: 10.1067/mtc.2002.122548 Copyright © 2002 American Association for Thoracic Surgery Terms and Conditions

Fig. 1 Change in resting force in porcine coronary microarteries during incubation with Krebs solution (control, subgroup Ia, filled circles) or solution containing 20-mmol/L potassium ion (subgroup Ib, open circles), 16-mmol/L magnesium ion (subgroup Ic, filled squares), or 20-mmol/L potassium ion plus 16 mmol/L magnesium ion (subgroup Id, open squares). Four rings taken from same microartery were allocated in four chambers of myograph, n = 8 in each group. Triple asterisk indicates P <.001 vs Krebs solution by ANOVA and Scheffé F test. The Journal of Thoracic and Cardiovascular Surgery 2002 124, 361-370DOI: (10.1067/mtc.2002.122548) Copyright © 2002 American Association for Thoracic Surgery Terms and Conditions

Fig. 2 Mean contraction force response to U46619 (range −8 to −7 log mol/L, see Methods section) after exposure to Krebs solution (control, subgroup Ia) or solution containing 20-mmol/L potassium ion (subgroup Ib, K20), 16-mmol/L magnesium ion (subgroup Ic, Mg16), or potassium ion plus magnesium ion (subgroup Id, K20+Mg16) for 1 hour in groups I (A) and III (B). Four rings taken from same microartery were allocated in four chambers of myograph, n = 8 in each group. A, P <.001 among 4 subgroups by ANOVA, but P >.05 for subgroups Ib, Ic, and Id compared with Krebs solution (control subgroup) by ANOVA and Scheffé F test. B, P >.05 among 4 subgroups. The Journal of Thoracic and Cardiovascular Surgery 2002 124, 361-370DOI: (10.1067/mtc.2002.122548) Copyright © 2002 American Association for Thoracic Surgery Terms and Conditions

Fig. 3 Concentration-relaxation curves for bradykinin (A, group I) and SNP (B, group II) in U46619-precontracted porcine coronary microarteries. Bradykinin-induced relaxation was performed with presence of indomethacin (7 μmol/L), l-NNA (300 μmol/L), and oxyhemoglobin (20 μmol/L) after exposure to following solutions for 1 hour: subgroup Ia or IIa (control, filled circles), Krebs solution; subgroup Ib or IIb (open circles), solution containing 20-mmol/L potassium ion; subgroup Ic or IIc (filled squares), solution containing 16-mmol/L magnesium ion; and subgroup Id or IId (open squares), solution containing 20-mmol/L potassium ion plus 16-mmol/L magnesium ion. Four rings taken from same microartery were allocated in four chambers of myograph; n = 8 in each subgroup. Asterisk indicates P <.05 compared with control subgroup by ANOVA and Scheffé F test; double asterisk indicates P <.01. The Journal of Thoracic and Cardiovascular Surgery 2002 124, 361-370DOI: (10.1067/mtc.2002.122548) Copyright © 2002 American Association for Thoracic Surgery Terms and Conditions

Fig. 4 Concentration-relaxation curves for bradykinin (group III) in U46619-precontracted porcine coronary microarteries with presence of indomethacin (7 μmol/L), l-NNA (300 μmol/L), and oxyhemoglobin (20 μmol/L) after exposure to following solutions for 1 hour followed by 45-minute washout: subgroup IIIa (control, filled circles), Krebs solution; subgroup IIIb (open circles), solution containing 20-mmol/L potassium ion; subgroup IIIc (filled squares), solution containing 16-mmol/L magnesium ion; and subgroup IIId (open squares), solution containing 20-mmol/L potassium ion plus 16-mmol/L magnesium ion. Four rings taken from same microartery were allocated in four chambers of myograph; n = 8 in each subgroup. The Journal of Thoracic and Cardiovascular Surgery 2002 124, 361-370DOI: (10.1067/mtc.2002.122548) Copyright © 2002 American Association for Thoracic Surgery Terms and Conditions

Fig. 5 Mean membrane potential of smooth muscle cells of porcine coronary arteries in presence of indomethacin (7 μmol/L) and l-NNA (300 μmol/L) after superfusion for 1 hour with solution containing 20-mmol/L potassium ion (subgroup IVa, n = 7, K20), solution containing 16-mmol/L magnesium ion (subgroup IVb, n = 6, Mg16), or solution containing 20-mmol/L potassium ion plus 16-mmol/L magnesium ion (subgroup IVc, n = 6, K20+Mg16). Triple asterisk indicates P <.001 versus control subgroup (before superfusion) by paired t test. The Journal of Thoracic and Cardiovascular Surgery 2002 124, 361-370DOI: (10.1067/mtc.2002.122548) Copyright © 2002 American Association for Thoracic Surgery Terms and Conditions

Fig. 6 Membrane potential measurement of smooth muscle cells of porcine coronary arteries. Concentration-response (membrane potential) curves for bradykinin (A-C) in presence of indomethacin (7 μmol/L) and l-NNA (300 μmol/L) after superfusion for 1 hour. A, Superfusion with solution containing 20-mmol/L potassium ion (subgroup IVa, n = 7, open circles). B, Superfusion with solution containing 16-mmol/L magnesium ion (subgroup IVb, n = 6, open circles). C, Superfusion with solution containing 20-mmol/L potassium ion plus 16 mmol/magnesium ion (subgroup IVc, n = 6, open circles). D, Change of membrane potential to bradykinin (−6.5 log mol/L). Asterisk indicates P <.05 vs control subgroup (before superfusion) by paired t test; double asterisk indicates P <.01; double pound sign indicates p <.01 vs 20-mmol/L potassium by unpaired t test. The Journal of Thoracic and Cardiovascular Surgery 2002 124, 361-370DOI: (10.1067/mtc.2002.122548) Copyright © 2002 American Association for Thoracic Surgery Terms and Conditions