IL-18; IL-18BP; and free, biologically active IL-18 levels in nonsevere versus severe dengue cases. IL-18; IL-18BP; and free, biologically active IL-18.

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IL-18; IL-18BP; and free, biologically active IL-18 levels in nonsevere versus severe dengue cases. IL-18; IL-18BP; and free, biologically active IL-18 levels in nonsevere versus severe dengue cases. Plasma levels of total interleukin-18 (IL-18) (A); IL-18 binding protein (IL-18BP) (B); and free, biologically active IL-18 (C) in Indonesian adults with nonsevere dengue fever (n = 65; n = 4 missing) and severe dengue fever (n = 12; n = 3 missing) during the critical phase. The minimum detectable concentration was 9.8 pg/ml for total IL-18 and 0.1 ng/ml for IL-18BP. The level of free, bioactive IL-18 was calculated based on the mass-action law, using a dissociation constant of 400 pM and a stoichiometric ratio of 1:1. The dots represent the individual measurements, the horizontal lines represent median values, and the whiskers represent interquartile ranges. P values were determined by Mann-Whitney U test for comparison of nonsevere dengue and severe dengue. A P value of <0.05 was considered significant. Meta Michels et al. Clin. Vaccine Immunol. 2015; doi:10.1128/CVI.00147-15