Presence of a 31-kD protein band in human cumulus–corona radiata–conditioned media and pregnancy outcome Sandra Cecconi, >Gianna Rossi, B.Sc., Roberto.

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Presentation transcript:

Presence of a 31-kD protein band in human cumulus–corona radiata–conditioned media and pregnancy outcome Sandra Cecconi, >Gianna Rossi, B.Sc., Roberto Gualtieri, Ph.D., Riccardo Talevi, Ph.D. Fertility and Sterility Volume 75, Issue 5, Pages 966-972 (May 2001) DOI: 10.1016/S0015-0282(01)01754-X

Figure 1 Representative micrographs of adhering CC cells. (a) Dark-field micrograph of adhering CC after zygote removal (arrow) at 19 hours after insemination; ×50. (b) HA CC cells. Note the well-packed polygonal cells; ×200. (c) Magnification of the leading edge of HA CC cells showing intense adhesive ability characterized by the presence of elongated pseudopodia; ×600. (d) LA CC cells. Cells appear more scattered and round shaped; ×200. Cecconi. Cumulus-corona cells and pregnancy rates. Fertil Steril 2001. Fertility and Sterility 2001 75, 966-972DOI: (10.1016/S0015-0282(01)01754-X)

Figure 2 Transmission electron micrographs of HA (a,b) and LA (c,d) CC cells (a,b) Typical ultrastructure of HA CC cells (a, ×4,000; b, ×11,000); the inset in a shows the boxed region at high magnification (×30,000). (c,d) Typical ultrastructure of LA CC cells (×4,000). Note in d the presence of cell debris. Cecconi. Cumulus-corona cells and pregnancy rates. Fertil Steril 2001. Fertility and Sterility 2001 75, 966-972DOI: (10.1016/S0015-0282(01)01754-X)

Figure 3 Representative depictions of sodium dodecyl sulfate–polyacrylamide gel electrophoresis of the CC-conditioned media after 24 hours of culture. Cells were obtained from grade 2 COCs. The fertilized oocytes developed into grade A embryos that failed (lane a) implantation or not (lane b). Arrow indicates 31-kD band. mw = molecular weight. Cecconi. Cumulus-corona cells and pregnancy rates. Fertil Steril 2001. Fertility and Sterility 2001 75, 966-972DOI: (10.1016/S0015-0282(01)01754-X)