Selenomethionine inhibits IL-1β inducible nitric oxide synthase (iNOS) and cyclooxygenase 2 (COX2) expression in primary human chondrocytes A.W.M. Cheng,

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Selenomethionine inhibits IL-1β inducible nitric oxide synthase (iNOS) and cyclooxygenase 2 (COX2) expression in primary human chondrocytes A.W.M. Cheng, T.V. Stabler, M. Bolognesi, V.B. Kraus Osteoarthritis and Cartilage Volume 19, Issue 1, Pages 118-125 (January 2011) DOI: 10.1016/j.joca.2010.10.019 Copyright © 2010 Osteoarthritis Research Society International Terms and Conditions

Fig. 1 MTT cell toxicity assay for optimization of in vitro chondrocyte culture conditions. Effects of Se on cell viability were assessed with the MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. (A) SW-1353 cells were cultured with different concentrations (0μM–5μM) of selenite or SeMet for 48h. (B) Primary human chondrocytes were cultured for 24h in the absence (control) or presence of 0.5μM SeMet, followed by 24h co-treatment without or with 50pg/ml IL-1β. Values shown are mean and SEM of cell viability as a percentage of control (set at 100%). Osteoarthritis and Cartilage 2011 19, 118-125DOI: (10.1016/j.joca.2010.10.019) Copyright © 2010 Osteoarthritis Research Society International Terms and Conditions

Fig. 2 SeMet inhibited IL-1β induced iNOS gene expression and NO production in primary human chondrocytes. Primary human chondrocytes were cultured for 24h in the absence (control) or presence of 0.5μM SeMet, followed by 24h co-treatment without IL-1β (control) or with either 10pg/ml (A and C) or 50pg/ml (B and D) IL-1β. Gene expression for iNOS (A and B) was determined by RT-PCR normalized to 18S rRNA (average of triplicates for six independent experiments). Corresponding culture media were analyzed for NO2− concentration (C and D). Data were normalized to total DNA of the corresponding chondrocytes. Values shown are mean and SEM of iNOS or NO2− as a percentage of control (set at 100%). *P=0.031. Osteoarthritis and Cartilage 2011 19, 118-125DOI: (10.1016/j.joca.2010.10.019) Copyright © 2010 Osteoarthritis Research Society International Terms and Conditions

Fig. 3 SeMet inhibited IL-1β induced COX2 gene expression and PGE2 production in primary human chondrocytes. Primary human chondrocytes were cultured for 24h in the absence (control) or presence of 0.5μM SeMet, followed by 24h co-treatment without IL-1β (control) or with either 10pg/ml (A and C) or 50pg/ml (B and D) IL-1β. Gene expression for COX2 (A and B) was determined by RT-PCR normalized to 18S rRNA (average of triplicates for six independent experiments). Corresponding culture media were analyzed for PGE2 concentration (C and D). Data were normalized to total DNA of the corresponding chondrocytes. Values shown are mean and SEM of COX2 or PGE2 as a percentage of control (set at 100%). *P=0.031. Osteoarthritis and Cartilage 2011 19, 118-125DOI: (10.1016/j.joca.2010.10.019) Copyright © 2010 Osteoarthritis Research Society International Terms and Conditions

Fig. 4 SeMet selectively blocked IL-1β induced p38 MAPK. (A) Western blot analysis. Primary human chondrocytes were cultured for 24h in the absence (control) or presence of 0.5μM SeMet, and then co-treated with 50pg/ml IL-1β treatment for 0, 5, 30 and 45min. Equal amounts of total cell lysate were separated by SDS-PAGE and analyzed by Western blot for the following proteins: Phospho-p38 MAPK (Thr180/Tyr182), Total p38 MAPK, Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204), Phospho-SAPK/JNK (Thr183/Tyr185), Phospho-IKKα/β (Ser176/180) and Phospho-NFκB p65 (Ser536); α tubulin (bottom row) was used as a control for normalization. The results shown are representative of three independent experiments. (B) Quantification of the effects of SeMet on IL-1β induced phosphorylation of signaling proteins. Phosphoprotein signaling intensity was quantified by Image J analysis of Western blots for up to three-time points from three independent experiments (as described in Fig. 3) with normalization to the internal control, α-tubulin. The normalized mean and SEM levels of phosphosignaling protein intensity are shown for Phospho-p38 MAPK (Thr180/Tyr182), Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204), Phospho-SAPK/JNK (Thr183/Tyr185), Phospho-IKKα/β (Ser176/180) and Phospho-NFκB p65(Ser536). SeMet inhibited IL-1β activation of p38 MAPK significantly but not the generation of the other phosphoproteins. *P=0.039. Osteoarthritis and Cartilage 2011 19, 118-125DOI: (10.1016/j.joca.2010.10.019) Copyright © 2010 Osteoarthritis Research Society International Terms and Conditions