(Life Sciences College, Nankai University )

Slides:

Advertisements

Similar presentations

Manipulating the Genome: DNA Cloning and Analysis 20.1 – 20.3 Lesson 4.8.

Advertisements

Primers. What is a primer? Primers are oligonucleotides, small pieces of RNA or DNA up to 30 base pairs long (a bigger piece is known as a polynucleotide)

Paras Yadav 1, Aarti Bhardwaj 3, Shalini Jain 2 and Hariom Yadav 2 1 Animal Biotechnology Division, National Dairy Research Institute, Karnal , Haryana,

Genetics and Biotechnology

TOPICS IN (NANO) BIOTECHNOLOGY Lecture 7 5th May, 2006 PhD Course.

From Haystacks to Needles AP Biology Fall Isolating Genes  Gene library: a collection of bacteria that house different cloned DNA fragments, one.

HAPLOID GENOME SIZES (DNA PER HAPLOID CELL) Size rangeExample speciesEx. Size BACTERIA1-10 Mb E. coli: Mb FUNGI10-40 Mb S. cerevisiae 13 Mb INSECTS.

AP Biology: Chapter 14 DNA Technologies

Genomic walking (1) To start, you need: -the DNA sequence of a small region of the chromosome -An adaptor: a small piece of DNA, nucleotides long.

Genome mapping. Techniques Used in the Human Genome Project 1.Linkage mapping can be used to locate genes on particular chromosomes and establish the.

How do you identify and clone a gene of interest? Shotgun approach? Is there a better way?

1 Meiosis and genetic variation. Introduction 2 3 Genes DNA is organized in chromosomes. –Genes have specific places on chromosomes.

DNA Technologies.

Recombinant Technololgy

Remember the limitations? –You must know the sequence of the primer sites to use PCR –How do you go about sequencing regions of a genome about which you.

©2000 Timothy G. Standish John 15:4 4Abide in me, and I in you. As the branch cannot bear fruit of itself, except it abide in the vine; no more can ye,

DNA Technology. Overview DNA technology makes it possible to clone genes for basic research and commercial applications DNA technology is a powerful set.

5.3 – Advances in Genetics Trashketball!. Selecting organisms with desired traits to be parents of the next generation is… A. Inbreeding A. Inbreeding.

Genetic Engineering Genetic engineering is also referred to as recombinant DNA technology – new combinations of genetic material are produced by artificially.

AP Biology Biotech Tools Review AP Biology Biotech Tools Review  Recombinant DNA / Cloning gene  restriction enzyme, plasmids,

University of Essex BIODEEP-WP3 Analysis of species diversity, community structures and phylogeny of microorganisms and meiofauna in the Mediterranean.

Chapter 20 DNA Technology and Genomics. Biotechnology is the manipulation of organisms or their components to make useful products. Recombinant DNA is.

Introduction to DNA. Question: From your on-line computer activity, what do you know about the structure of DNA?

 Genetics Primer: SBI 4UI Mrs. Tuma. Test Your Genetic IQ: 1. The Human Genome contains 3 billion base pairs. True or False?

Figure 1. Transposon-tagged mutant chromosome (cmsm). WT S. typhimurium cells (WT cmsm on left) were mutagenized by introducing a transposon (Tn) that.

Meiosis 2n n n = number of chromosome Diploid: cells that have two copies of every chromosome Haploid: cells that have one copy of every chromosome Meiosis.

DO NOW What is a genome? In what year was the Human Genome Sequence completed? How different is your genome from Mrs. Schwichtenberg? (Give a percent)

DNA Fingerprinting Maryam Ahmed Khan February 14, 2001.

DNA FINGERPRINTING. Monday Jan 25 SW describe how techniques such as DNA fingerprinting, genetic modifications, and chromosomal analysis are used to study.

An introduction of TAIL PCR

Detecting DNA with DNA probes arrays. DNA sequences can be detected by DNA probes and arrays (= collection of microscopic DNA spots attached to a solid.

Topic Cloning and analyzing oxalate degrading enzymes to see if they dissolve kidney stones with Dr. VanWert.

2470 bp 1891 bp WT bp 2314 bp A B Fig. S1. Verification with PCR amplification of the.

Immunohistochemistry and in situ hybridization allow researchers to pinpoint the expression of their protein and nucleic acid targets, respectively.

Quantitative Detection and Differentiation of Human Herpesvirus 6 Subtypes in Bone Marrow Transplant Patients by Using a Single Real-Time Polymerase Chain.

Genomic and cDNA Libraries

Pre-genomic era: finding your own clones

Figure 2. PFGE and Southern blot hybridization analysis results for E

Section 2 Genetics and Biotechnology DNA Technology

Lisa Edelmann, Raj K. Pandita, Bernice E. Morrow

Fig. S Fig. S2 Cre-mediated recombination in vivo. G2 mice displaying high levels of GFP were crossed.

Biotech Tools Review

the manipulation of living organisms for human use Chapter 13

Lab 8: PTC Polymerase Chain Reaction Lab

Vav‐1 gene‐targeting strategy.

KEY CONCEPT Entire genomes are sequenced, studied, and compared.

by David M. Weinstock, Beth Elliott, and Maria Jasin

Brca1 Controls Homology-Directed DNA Repair

Volume 101, Issue 5, Pages (May 2000)

Human Epidermal Differentiation Complex in a Single 2

Genetics and Biotechnology

Evolutionary Origin of the Medaka Y Chromosome

A Novel Family of Candidate Pheromone Receptors in Mammals

HUMAN HEREDITY.

Volume 2, Issue 2, Pages (August 1998)

Fluorescence Imaging of Single-Copy DNA Sequences within the Human Genome Using PNA-Directed Padlock Probe Assembly Anastasia I. Yaroslavsky, Irina V.

Amplification and Overexpression of the EMS 1 Oncogene, a Possible Prognostic Marker, in Human Hepatocellular Carcinoma Bao-Zhu Yuan, Xiaoling Zhou,

Gayatry Mohapatra, Rebecca A. Betensky, Ezra R

Sex-Linked period Genes in the Silkmoth, Antheraea pernyi

KEY CONCEPT Entire genomes are sequenced, studied, and compared.

APOE Gene Targeting (A) Schematic representation of the endogenous APOE locus, the gene targeting vector and the targeted APOE locus. The exons of the.

Identification of candidate genes within the 1q32 locus.

Volume 7, Issue 1, Pages (January 2001)

Fig. 4. Targeted disruption of STK35 transcripts in mouse.

Expression of multiple forms of MEL1 gene products.

Whole-chromosome view of gene density, inverse environmental variation, inverse total variation, and open chromatin signature. Whole-chromosome view of.

KEY CONCEPT Entire genomes are sequenced, studied, and compared.

Southern blot demonstrating allelic heterozygosity of the vspC5 gene as well as members of the family of genes related to vspC5. Southern blot demonstrating.

Volume 84, Issue 2, Pages (January 1996)

Presentation transcript:

(Life Sciences College, Nankai University ) The cloning of the Brine Shrimp DNA fragments correlated to the Bombyx mori doublesex gene Zeng Hui (Life Sciences College, Nankai University )

>1.0 0.5

The sex-determination system differs extremely among organisms. sex chromosomes , XX/XY of ZZ/ZW autosomal genes , (diploid/haploid sex-determination system ) environmental and other epigenetic factors However, the study of the dsx gene has shown an apparent exception.

The homologues of dsx have been isolated in the silkworm, mouse, human, chicken and other insects. The mab-3 gene of Caenorhabditis elegans is also the structural homologue of dsx. Mouse,chicken Human Nematode Silkworm mab-3 Bmdsx Dmrt1 DMRT1 homologues of dsx

The following amplification showed that this pair of primers could make two visible bands appear from genomic DNA of Artemia parthenogenetica from Gahai Lake, China. The two bands were named Apdsx900 and Apdsx200 respectively. 1 2 3 4 1. DNA 1000bp~100bp ladder, 2. genomic DNA of Artemia sinica , 3. genomic DNA of Artemia parthenogenetica, 4. λDNA/Hind III+EcoR I Apdsx900 Apdsx200

1. Artemia parthenogenetica, 2. Artemia sinica Apdsx200 were labeled as probe with DIG and hybridized respectively to the genomic DNA of Artemia parthenogenetica and Artemia sinica digested by restriction endonucease HaeIII. 1. Artemia parthenogenetica, 2. Artemia sinica The signals indicated that the Apdsx200 was a low copy DNA sequence.

The sequences of Apdsx900

Further analysis was required to assure the expression of the sequence of Apdsx900 in Artemia parthenogenetica bodies and localize it onto chromosomes of Artemia parthenogenetica.

Thank you very much!