Rapid field detection assays for Bacillus anthracis, Brucella spp

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Presentation transcript:

Rapid field detection assays for Bacillus anthracis, Brucella spp Rapid field detection assays for Bacillus anthracis, Brucella spp., Francisella tularensis and Yersinia pestis P. Matero, H. Hemmilä, H. Tomaso, H. Piiparinen, K. Rantakokko-Jalava, L. Nuotio, S. Nikkari Clinical Microbiology and Infection Volume 17, Issue 1, Pages 34-43 (January 2011) DOI: 10.1111/j.1469-0691.2010.03178.x Copyright © 2011 European Society of Clinical Infectious Diseases Terms and Conditions

Fig. 1 A representative Bacillus thuringiensis real-time PCR amplification plot obtained with the RAZOR instrument to test the specificity of the optimized primers (target gene cryT). Four different Bacillus strains (ELMI 21, ELMI 44, ELMI 123 and ELMI 325) were analysed. As the positive control, 400 pg of chromosomal DNA from the TUREX insecticide powder was used per reaction. No template controls (NTCs) were included as negative controls. All assays were performed in duplicate, using the 6 × 2 pouches. Both the positive control (amplification curves 1 and 2) and the B. thuringiensis strain (ELMI 123; amplification curves 3 and 4) showed amplification, the average Ct values being 17.1 and 21.6, respectively. The other Bacillus strains tested (ELMI 21, ELMI 44 and ELMI 325), as well as the NTCs, were negative. Clinical Microbiology and Infection 2011 17, 34-43DOI: (10.1111/j.1469-0691.2010.03178.x) Copyright © 2011 European Society of Clinical Infectious Diseases Terms and Conditions

Fig. 2 Analytical test results from the field trial with the RAZOR instrument. Positive controls (amplification curves 1 and 2) and Bacillus thuringiesis-positive samples (amplification curves 3 and 4, containing 0.05 g of TUREX insecticide combined with 0.5 g of rye flour, and amplification curves 5 and 6, containing 0.005 g of TUREX insecticide combined with 0.5 g of rye flour) showed the expected positive fluorescence signals. Neither the negative samples nor the non-template control showed any PCR reactivity. Clinical Microbiology and Infection 2011 17, 34-43DOI: (10.1111/j.1469-0691.2010.03178.x) Copyright © 2011 European Society of Clinical Infectious Diseases Terms and Conditions