In vitro quantification of exon skipping associated with 15 premature termination codons using minigenes. In vitro quantification of exon skipping associated with 15 premature termination codons using minigenes. The percentage of exon skipping measured by reverse transcriptase-PCR with minigenes containing wild-type exons or indicated mutated exons is shown. #: significant increased exon skipping (p<0.001, unpaired t-test, n=5–10). Iwona Pranke et al. ERJ Open Res 2018;4:00080-2017 ©2018 by European Respiratory Society