L. Gato-Calvo, Á. Vela-Anero, E. Loures-Fraga, C. Ruiz Romero, F. J

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Proliferative and Anti-Inflammatory Effect of Platelet-Rich Plasma on Osteoarthritic Chondrocytes L. Gato-Calvo, Á. Vela-Anero, E. Loures-Fraga, C. Ruiz Romero, F.J. Blanco, E.F. Burguera Osteoarthritis and Cartilage Volume 25, Pages S270-S271 (April 2017) DOI: 10.1016/j.joca.2017.02.456 Copyright © 2017 Terms and Conditions

Figure 1. Chondrocyte proliferation in DMEM 10% FBS (Basal) and DMEM 0% + 10% PRPr according to cell count (a), protein count (b) and cell activity measured with alamarBlue (c). Cell counts were 0.3–2.6 times bigger in PRPr-supplemented medium. Similar trends were found in protein counts and cell activity measurements. *p-value < 0.05, **p-value < 0.01. Osteoarthritis and Cartilage 2017 25, S270-S271DOI: (10.1016/j.joca.2017.02.456) Copyright © 2017 Terms and Conditions

Figure 2. Inflammatory response of chondrocytes after 48 h of co-estimulation with IL-1B and PRPr. Optical microscopy (a) shows the recovery of basal morphology under the effect of PRPr. Pro-inflammatory gene markers (b) were downregulated by 60–80% on average in knee CH, with top reductions of 95–99% for iNOS and MMP-13. Data are expressed as mean ± SEM. ***p-value < 0.0001. Osteoarthritis and Cartilage 2017 25, S270-S271DOI: (10.1016/j.joca.2017.02.456) Copyright © 2017 Terms and Conditions