Fig. 1. Experimental workflow of the dAST method and computationally estimated operational space. Experimental workflow of the dAST method and computationally.

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Fig. 1. Loss of circadian rhythms in iKO mice.

Fig. 2. Effects of AZD4785 on proliferation and MAPK pathway signaling in KRAS mutant and wild-type cancer cells in vitro. Effects of AZD4785 on proliferation.

Fig. 1. Generation of ERY974. Generation of ERY974. (A) Schematic illustration of ERY974 structure and the introduced mutations. The two Fab arms share.

Fig. 5. Protective efficacy of the combination of PGT121 + PGDM1400 against a mixed SHIV challenge in rhesus monkeys. Protective efficacy of the combination.

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Fig. 6. dAST directly from clinical samples using dPCR and dLAMP for quantification. dAST directly from clinical samples using dPCR and dLAMP for quantification.

Fig. 3. Cytokines trigger proliferation and block differentiation of perivascular adipocytes. Cytokines trigger proliferation and block differentiation.

Fig. 1. The cell-intrinsic fibroblast circadian proteome contains numerous cytoskeletal regulators. The cell-intrinsic fibroblast circadian proteome contains.

Fig. 5. Circulating PPi concentration does not correlate with severity of calcification phenotype in mice. Circulating PPi concentration does not correlate.

Fig. 3 Urinary LAM concentration predicted pulmonary TB and correlated to mycobacterial burden and weight loss. Urinary LAM concentration predicted pulmonary.

Fig. 2. Mutational signature exposures in Taiwan HCCs and summary of AA signature mutations. Mutational signature exposures in Taiwan HCCs and summary.

Fig. 2. Bestatin treatment improves tail anatomy and restores lymphatic function. Bestatin treatment improves tail anatomy and restores lymphatic function.

Fig. 4 Bacterial taxonomic groups that discriminate among RYGB-, SHAM-, and WMS-derived samples. Bacterial taxonomic groups that discriminate among RYGB-,

Fig. 3. Antimicrobial activity is detected in diverse strains of CoNS and not predictable at the species level. Antimicrobial activity is detected in diverse.

Fig. 5. Correlation of tail and long bone growth velocities with Cxm serum concentrations in mice. Correlation of tail and long bone growth velocities.

Fig. 4. Liver HBV mRNA paired-end sequencing reads in HBeAg-positive and HBeAg-negative chimpanzees. Liver HBV mRNA paired-end sequencing reads in HBeAg-positive.

Fig. 4. Specific antibiotics reduce fecal biomass in mice and humans.

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Fig. 2. dAST using dPCR is robust to the presence of high concentrations of commensal bacteria due to the specificity of NA amplification. dAST using dPCR.

Fig. 2. In vitro profiling of tEV markers on cell line–derived EVs.

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Fig. 2 TLR8 is aberrantly expressed on pDCs from SSc patients.

Fig. 3. Frequencies of amino acids at critical PGT121 and contact sites in the SHIV-SF162P3 challenge stock. Frequencies of amino acids at critical.

Fig. 6 Bimodal treatment results in reduced tinnitus loudness and reduced TFI scores in human patients. Bimodal treatment results in reduced tinnitus loudness.

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Fig. 5. MasSpec Pen analysis of an HGSC tissue sample with mixed histologic composition. MasSpec Pen analysis of an HGSC tissue sample with mixed histologic.

Fig. 4 Nanocages captured multiple TB-related analytes.

Fig. 4 Phylogeny of rabies strains isolated during and after the mass vaccination campaign. Phylogeny of rabies strains isolated during and after the mass.

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Fig. 7 Human study design for device testing.

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Fig. 2. Pathways differentially regulated in patients with early Lyme disease and STARI. Pathways differentially regulated in patients with early Lyme.

Workflow of a sample-to-answer AST performed in less than 30 min

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Fig. 3. H3N2 incidence forecasts based on the cluster model for the Unites States. H3N2 incidence forecasts based on the cluster model for the Unites States.

Fig. 2 Analysis of CLL lymph nodes.

Fig. 2. IONP characterization.

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Fig. 6. Analysis of SHIV-325c V1V2 envelope sequences in breakthrough infections. Analysis of SHIV-325c V1V2 envelope sequences in breakthrough infections.

Fig. 2. Exposure of both TCR and CAR antigens diminishes efficacy of CAR8 but not CAR4 cells. Exposure of both TCR and CAR antigens diminishes efficacy.

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Fig. 2. BET inhibition enhances PARPi-induced DNA damage.

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Fig. 1. Detection of circulating tumor DNA in CRPC patients.

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Presentation transcript:

Fig. 1. Experimental workflow of the dAST method and computationally estimated operational space. Experimental workflow of the dAST method and computationally estimated operational space. (A) The workflow for detecting antibiotic susceptibility by measuring the quantity of a specific NA sequence (AST marker). Urine samples are incubated without and with antibiotics (ABX) (steps 1 and 2), AST markers are quantified in control (−ABX) and treated (+ABX) samples (step 3), and the CT ratios are analyzed (step 4). (B) Theoretical model that predicts a CT ratio as a function of pathogen DNA doubling time and antibiotic exposure time. The operational space gained by using digital counting compared with qPCR is outlined in red. Nathan G. Schoepp et al., Sci Transl Med 2017;9:eaal3693 Published by AAAS