Nelson, PT_Suppl_Fig2 A B.

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Nelson, PT_Suppl_Fig2 A B

* ** ** Lysate Co-IP after transfections Nelson, PT_Suppl_Fig3 GRN GRN BACE1 BACE1 n=3 biological replicates each MiR-107 v NEG-CTRL *-p<0.01; **-p<0.0001

A B C D Anti-AGO co-IP with downstream qPCR Nelson, PT_Suppl_Fig4 A 13 miR-107* 35 c c --c u u c u a ucu ugcuuu agcu cu uacaguguugc uug ggc u ||| |||||| |||| || ||||||||||| ||| ||| g aga acgaaa ucgg ga auguuacgacg aac uug g c cua - c - - a 72 miR-107 50 5’ 3’ Homo sapiens miR-107 stem-loop B u u c agcu cu uacaguguugc uug miR-107* |||| || ||||||||||| | ucgg ga auguuacgacg a miR-107 acua - c - 5’ 3’ 5’ 3’ miR-107 duplex for transfection C D Anti-AGO co-IP with downstream qPCR

A B Y axis: # miR-16 seed sequences/1000 nts in 5’utr, CDS, and 3’UTR Nelson, PT_Suppl_Fig5 Y axis: # miR-16 seed sequences/1000 nts in 5’utr, CDS, and 3’UTR A X axis: miR-16 targets sorted by enrichment in AGO co-IP (50 mRNAs/grp) B 1 1000 2000 3000 X axis: miR-16 targets sorted by mRNA lysate “knock-down” (50 mRNAs/grp) 1 1000 2000 3000