Functional characteristics of neutrophils prior to and following (phorbol myristate acetate) PMA stimulation. Functional characteristics of neutrophils prior to and following (phorbol myristate acetate) PMA stimulation. (A) Neutrophil fluorescence intensity; (B) cell size (forward scatter (FSC-H)); (C) granularity (side scatter (SSC-H)); (D) stimulation index (SI) ((mean channel fluorescence time B − mean channel fluorescence time A) / (mean channel fluorescence time A)) before (0 min) and after (5 min, 10 min, 15 min) PMA stimulation. Values are presented as mean ±SE. * indicates mean within each time point is significantly different from the stage 0 obesity group (p<0.05). Caroline Richard et al. BMJ Open Diab Res Care 2017;5:e000379 ©2017 by American Diabetes Association