Intraoperative transfection of vein grafts with the NFκB decoy in a canine aortocoronary bypass model: a strategy to attenuate intimal hyperplasia Takuji.

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Intraoperative transfection of vein grafts with the NFκB decoy in a canine aortocoronary bypass model: a strategy to attenuate intimal hyperplasia Takuji Shintani, MD, Yoshiki Sawa, MD, Toshiki Takahashi, MD, Goro Matsumiya, MD, Nariaki Matsuura, MD, Yuji Miyamoto, MD, Hikaru Matsuda, MD The Annals of Thoracic Surgery Volume 74, Issue 4, Pages 1132-1137 (October 2002) DOI: 10.1016/S0003-4975(02)03921-8

Fig 1 Localization of fluorescent isothiocyanate (FITC)-labeled oligodeoxynucleotides (ODNs). Concentrated green FITC-labeled ODN signals are indicated in this fluorescence photomicrograph. ODNs are seen in the nuclei of the media and intima. Arrows indicate FITC-labeled ODNs in the nuclei of the media (×100 magnification). The numbers of FITC-positive and hematoxylin-positive nuclei were counted by using the public-domain image processing and analysis program (image, × 100 magnification). The average transfection efficiency of the decoy on the cross-section, defined as the ratio of the number of FITC-positive nuclei to the total number of nuclei based on hematoxylin staining, was 77% ± 20%. The Annals of Thoracic Surgery 2002 74, 1132-1137DOI: (10.1016/S0003-4975(02)03921-8)

Fig 2 Cross-sections of the middle portion of vein grafts.Arrows indicate the internal elastic lamina. The medial and intimal areas were calculated by using the computerized image analysis program, image. In comparison with that of the scrambled decoy group (a), neointimal hyperplasia of the NFκB decoy group (b) was significantly suppressed. (Hematoxylin & eosin staining, ×40 magnification.) (I= intima;M= media.) The Annals of Thoracic Surgery 2002 74, 1132-1137DOI: (10.1016/S0003-4975(02)03921-8)

Fig 3 α-actin staining.Arrows indicate the internal elastic lamina. α-actin was stained brown immunohistochemically by using the immunoperoxidase avidin-biotin complex system with nickel chloride color modification. Transfection of NFκB decoy tended to suppress the proliferation of the smooth muscle cells in the media. (a) Scrambled decoy group. (b) NFκB decoy group. (×200 magnification.) (I = intima;M = media.) The Annals of Thoracic Surgery 2002 74, 1132-1137DOI: (10.1016/S0003-4975(02)03921-8)

Fig 4 Extracellular matrix staining. Arrows indicate the internal elastic lamina. In Masson-trichrome staining, the extracellular matrix is stained blue. (a) Scrambled decoy group. In the (b) NFκB decoy group, suppression of superfluous synthesis of the extracellular matrix in the neointima was seen. (×100 magnification.) (I = intima;M= media.) The Annals of Thoracic Surgery 2002 74, 1132-1137DOI: (10.1016/S0003-4975(02)03921-8)