Ovarian reserve determinations suggest new function of FMR1 (fragile X gene) in regulating ovarian ageing  Norbert Gleicher, Andrea Weghofer, David H. Barad  Reproductive BioMedicine Online  Volume 20, Issue 6, Pages 768-775 (June 2010) DOI: 10.1016/j.rbmo.2010.02.020 Copyright © 2010 Reproductive Healthcare Ltd. Terms and Conditions

Figure 1 Box and whisker plot, defining normal range of CGG repeats for the whole study population of 339 women, reconfirming the previously defined normal range of 26–34 CGG repeats (median 30) (Gleicher et al., 2010). The graph in the right lower corner represents the frequency distribution of individual alleles, confirming the median CGG count at 30. Reproductive BioMedicine Online 2010 20, 768-775DOI: (10.1016/j.rbmo.2010.02.020) Copyright © 2010 Reproductive Healthcare Ltd. Terms and Conditions

Figure 2 Box and whisker plot, defining normal AMH concentrations and outliers based on zygosity in (A) egg donors and (B) infertility patients age <38years when physiological declines in ovarian reserve due to advancing age, can be expected to have only minor effects. *=one allele in normal count range (26–34) and one allele outside of normal range; **=both alleles outside of normal range. Bold horizontal lines in each box are median values. Amongst donors, no differences were noted. Younger infertility patients differed significantly amongst themselves (P=0.032) and homozygous women demonstrated significantly lower AMH than normals (P=0.009). Reproductive BioMedicine Online 2010 20, 768-775DOI: (10.1016/j.rbmo.2010.02.020) Copyright © 2010 Reproductive Healthcare Ltd. Terms and Conditions

Figure 3 Linear regression of association between AMH concentrations and age based on FMR1 status for egg donors and infertility patients at all ages. Reproductive BioMedicine Online 2010 20, 768-775DOI: (10.1016/j.rbmo.2010.02.020) Copyright © 2010 Reproductive Healthcare Ltd. Terms and Conditions

Figure 4 AMH concentrations in stratified age groups based on FMR1 status for egg donors and infertility patients of all ages. Under age 30years, AMH concentrations significantly differ amongst all three patient groups (P=0.009). Specifically, AMH is significantly higher between normal women and homozygous females (P<0.001) and between heterozygous and homozygous patients (P=0.002). By age 34.9, these statistical differences are no longer present. Reproductive BioMedicine Online 2010 20, 768-775DOI: (10.1016/j.rbmo.2010.02.020) Copyright © 2010 Reproductive Healthcare Ltd. Terms and Conditions