Role of hemodynamic forces in the ex vivo arterialization of human saphenous veins  Xavier Berard, MD, PhD, Sébastien Déglise, MD, Florian Alonso, PhD,

Slides:



Advertisements
Similar presentations
Ultrasound-guided percutaneous delivery of tissue-engineered endothelial cells to the adventitia of stented arteries controls the response to vascular.
Advertisements

Dipeptidyl peptidase 4 inhibitor reduces intimal hyperplasia in rabbit autologous jugular vein graft under poor distal runoff  Akio Koyama, MD, Kimihiro.
Inhibition of smooth muscle cell migration and neointima formation in vein grafts by overexpression of matrix metalloproteinase-3  Klaus Kallenbach, MD,
Mesenchymal stem cells attenuate angiotensin II-induced aortic aneurysm growth in apolipoprotein E-deficient mice  Ryotaro Hashizume, MD, Aika Yamawaki-Ogata,
Regulation of vascular smooth muscle cell expression and function of matrix metalloproteinases is mediated by estrogen and progesterone exposure  Oscar.
Reduced hind limb ischemia-reperfusion injury in Toll-like receptor-4 mutant mice is associated with decreased neutrophil extracellular traps  Rahmi Oklu,
In vivo electroporation of constitutively expressed HIF-1α plasmid DNA improves neovascularization in a mouse model of limb ischemia  Geoffrey O. Ouma,
Undifferentiated mesenchymal stem cells seeded on a vascular prosthesis contribute to the restoration of a physiologic vascular wall  Alain Mirza, MD,
Yi-kuan Chen, MD, Xue-mei Jiang, MSc, Jian-ping Gong, MD 
Elevation of hemopexin-like fragment of matrix metalloproteinase-2 tissue levels inhibits ischemic wound healing and angiogenesis  April E. Nedeau, MD,
Sustained orbital shear stress stimulates smooth muscle cell proliferation via the extracellular signal-regulated protein kinase 1/2 pathway  Hidenori.
Xue Ma, MD, Jeffrey D. Pearce, MD, David B. Wilson, MD, William P
Volume 85, Issue 2, Pages (January 2014)
Coarctation-induced degenerative abdominal aortic aneurysm in a porcine model  Pao-Yen Lin, MD, Yeng-Ting Wu, MS, Guan-Cheng Lin, BS, Yao Hsiang Shih,
Transglutaminase type 2 in human abdominal aortic aneurysm is a potential factor in the stabilization of extracellular matrix  Sung Shin, MD, Yong-Pil.
Fariba Chalajour, MD, Laura A
Reconstruction of pulmonary artery with porcine small intestinal submucosa in a lamb surgical model: Viability and growth potential  Lorenzo Boni, MD,
Pressure distention compared with pharmacologic relaxation in vein grafting upregulates matrix metalloproteinase-2 and -9  Ada W.Y. Chung, PhD, Pooja.
Tissue engineering applications to vascular bypass graft development: The use of adipose-derived stem cells  Paul DiMuzio, MD, Thomas Tulenko, PhD  Journal.
Effect of blocking platelet activation with AZD6140 on development of abdominal aortic aneurysm in a rat aneurysmal model  Jianping Dai, MD, PhD, Liliane.
Inflammation-related induction of absent in melanoma 2 (AIM2) in vascular cells and atherosclerotic lesions suggests a role in vascular pathogenesis 
Dipeptidyl peptidase 4 inhibitor reduces intimal hyperplasia in rabbit autologous jugular vein graft under poor distal runoff  Akio Koyama, MD, Kimihiro.
Lipoplex gene transfer of inducible nitric oxide synthase inhibits the reactive intimal hyperplasia after expanded polytetrafluoroethylene bypass grafting 
Blood-derived smooth muscle cells as a target for gene delivery
Association of smooth muscle cell phenotypes with extracellular matrix disorders in thoracic aortic dissection  Lixin Wang, MD, PhD, Jing Zhang, MD, PhD,
Lucas P. Neff, MD, Bryan W. Tillman, MD, PhD, Saami K
Controlled release of ascorbic acid from gelatin hydrogel attenuates abdominal aortic aneurysm formation in rat experimental abdominal aortic aneurysm.
Renal artery stenosis and aneurysms associated with neurofibromatosis
Α1-Adrenergic receptors mediate combined signals initiated by mechanical stretch stress and norepinephrine leading to accelerated mouse vein graft atherosclerosis 
Adenovirus-mediated intra-arterial delivery of cellular repressor of E1A-stimulated genes inhibits neointima formation in rabbits after balloon injury 
Increased connexin43 expression in human saphenous veins in culture is associated with intimal hyperplasia  Sébastien Déglise, MD, David Martin, MS, Hervé.
Orally administered dipeptidyl peptidase-4 inhibitor (alogliptin) prevents abdominal aortic aneurysm formation through an antioxidant effect in rats 
Long-term patency of small-diameter vascular graft made from fibroin, a silk-based biodegradable material  Soichiro Enomoto, MD, PhD, Makoto Sumi, MD,
Thomas E. Arnold, MD, Dmitri Gnatenko, PhD, Wadie F. Bahou, MD 
Pioglitazone preserves vein graft integrity in a rat aortic interposition model  Zhi Chen, MD, Tomomi Hasegawa, MD, PhD, Akiko Tanaka, MD, Yutaka Okita,
Daniel J. Wong, MD, Daniel Y. Lu, MD, Clinton D
Tissue factor pathway inhibitor-2 is induced by fluid shear stress in vascular smooth muscle cells and affects cell proliferation and survival  Johan.
Alterations in wall tension and shear stress modulate tyrosine kinase signaling and wall remodeling in experimental vein grafts  Tam T.T. Huynh, MD, Mark.
Matrix metalloproteinases modulated by protein kinase Cε mediate resistin-induced migration of human coronary artery smooth muscle cells  Qinxue Ding,
Mechanosensitive transient receptor potential vanilloid type 1 channels contribute to vascular remodeling of rat fistula veins  Yih-Sharng Chen, MD, PhD,
Recanalization of arterial thrombus, and inhibition with β-radiation in a new murine carotid occlusion model: mRNA expression of angiopoietins, metalloproteinases,
Connexin40 regulates renin production and blood pressure
Conformational stress and anastomotic hyperplasia
In vivo suppression of vein graft disease by nonviral, electroporation-mediated, gene transfer of tissue inhibitor of metalloproteinase-1 linked to the.
Ultrasound-guided percutaneous delivery of tissue-engineered endothelial cells to the adventitia of stented arteries controls the response to vascular.
Surgical marking pen dye inhibits saphenous vein cell proliferation and migration in saphenous vein graft tissue  Shinsuke Kikuchi, MD, Richard D. Kenagy,
Survivin expression is up-regulated in vascular injury and identifies a distinct cellular phenotype  Hector F. Simosa, MD, Grace Wang, MD, XinXin Sui,
The fate of an endothelium layer after preconditioning
Comparison of cell-type-specific vs transmural aortic gene expression in experimental aneurysms  Eiketsu Sho, MD, PhD, Mien Sho, MD, Hiroshi Nanjo, MD,
The temporal relationship between the development of vein graft intimal hyperplasia and growth factor gene expression  John R. Hoch, MD, Vida K. Stark,
Prolonged increases in vein wall tension increase matrix metalloproteinases and decrease constriction in rat vena cava: Potential implications in varicose.
Extracorporeal shock wave therapy ameliorates secondary lymphedema by promoting lymphangiogenesis  Masayuki Kubo, PhD, Tao-Sheng Li, MD, PhD, Takahiro.
Distinct macrophage phenotype and collagen organization within the intraluminal thrombus of abdominal aortic aneurysm  Jayashree Rao, MS, Bryan N. Brown,
Losartan ameliorates “upstream” pulmonary vein vasculopathy in a piglet model of pulmonary vein stenosis  Jiaquan Zhu, MD, PhD, Haruki Ide, MD, Yaqin.
The role of short-term oxygen administration in the prevention of intimal hyperplasia  Charu Lata, MBBS, Derrick Green, MD, Jing Wan, MD, Sabita Roy, PhD,
Lisheng Zhang, MD, Leigh Brian, MS, Neil J. Freedman, MD 
Morphologic characteristics of varicose veins: possible role of metalloproteinases  Kenneth J Woodside, MD, Mingdao Hu, MD, Ann Burke, MS, Maki Murakami,
In vitro differences between smooth muscle cells derived from varicose veins and normal veins  Ying Xiao, PhD, Zhibin Huang, MD, Henghui Yin, PhD, Ying.
Cathepsin G deficiency reduces periaortic calcium chloride injury-induced abdominal aortic aneurysms in mice  Jing Wang, MD, PhD, Galina K. Sukhova, PhD,
Metalloproteinase expression in venous aneurysms
Neointimal hyperplasia on a cell-seeded polytetrafluoroethylene graft is promoted by transfer of tissue plasminogen activator gene and inhibited by transfer.
Deep vein thrombosis resolution is impaired in diet-induced type 2 diabetic mice  Fatiha Bouzeghrane, PhD, Xiaochun Zhang, MD, BSc, Guylaine Gevry, BSc,
Richard L. Binns, MD  Journal of Vascular Surgery 
Identification and characterization of microRNAs in vascular smooth muscle cells from patients with abdominal aortic aneurysms  Bernice Lai Yee Cheuk,
Short-term dexamethasone treatment inhibits vein graft thickening in hypercholesterolemic ApoE3Leiden transgenic mice  Abbey Schepers, MD, Nuno M.M. Pires,
Controlled release of small interfering RNA targeting midkine attenuates intimal hyperplasia in vein grafts  Hiroshi Banno, MD, Yoshifumi Takei, PhD,
Differential transcriptional activation of matrix metalloproteinase-2 and membrane type-1 matrix metalloproteinase by experimental deep venous thrombosis.
Angiotensin II induces histomorphologic features of unstable plaque in a murine model of accelerated atherosclerosis  Valdeci da Cunha, PhD, Baby Martin-McNulty,
Remodeling of experimental arteriovenous fistula with increased matrix metalloproteinase expression in rats  Chih-Yang Chan, MD, Yih-Sharng Chen, MD,
Prevention of stenosis after vascular reconstruction: Pharmacologic control of intimal hyperplasia—A review  Alexander W. Clowes, MD, Michael A. Reidy,
Presentation transcript:

Role of hemodynamic forces in the ex vivo arterialization of human saphenous veins  Xavier Berard, MD, PhD, Sébastien Déglise, MD, Florian Alonso, PhD, François Saucy, MD, Paolo Meda, MD, Laurence Bordenave, MD, PhD, Jean-Marc Corpataux, MD, Jacques-Antoine Haefliger, PhD  Journal of Vascular Surgery  Volume 57, Issue 5, Pages 1371-1382 (May 2013) DOI: 10.1016/j.jvs.2012.09.041 Copyright © 2013 Society for Vascular Surgery Terms and Conditions

Fig 1 Intimal hyperplasia develops within 7 days of ex vivo pulsatile perfusion. Upper panel, Representative histological sections show the normal intima thickness (arrow) in nonperfused veins (control) and veins perfused at low pressure (arterial shear stress [SS] 7 mm Hg). In contrast, intimal hyperplasia (arrow) is evident in veins perfused at high pressure (arterial SS 70 mm Hg). L, Lumen; M, media. Bar represents 50 μm. Lower panels, Measurements of intima thickness show that intimal hyperplasia was induced by perfusion at high pressure (70 mm Hg), despite unchanged thickness of the media layer. Data represent mean ± standard error of the mean of two series of eight and nine experiments. *P < .05 and ***P < .001 vs the nonperfused segments (controls) of the same veins. Journal of Vascular Surgery 2013 57, 1371-1382DOI: (10.1016/j.jvs.2012.09.041) Copyright © 2013 Society for Vascular Surgery Terms and Conditions

Fig 2 Human saphenous veins after 7 days of ex vivo perfusion show persistence of endothelial cells and altered distribution of smooth muscle cells (SMCs). Upper row, Hematoxylin-eosin staining (HE) reveals the lining of the lumen by nuclei of endothelial cells and the nuclei of SMCs in the media layer. Nuclei of the latter cells were also observed within the intimal hyperplasia regions (right panel). Middle rows, Immunolabeling using antibodies against von Willebrand factor (vWf) or alpha-smooth muscle actin (SMA) demonstrate the presence of endothelial cells at the luminal surface of all veins and of SMCs in the media of all vessels. SMCs were also detected in the intimal hyperplasia regions (right panels). Lower row, Immunolabeling using antibodies against KI-67 (dark blue) revealed the presence of proliferating SMCs mainly in the intimal hyperplasia regions. L, Lumen; M, media. Bars represent 100 μm in figures and 400 μm in the insets. Journal of Vascular Surgery 2013 57, 1371-1382DOI: (10.1016/j.jvs.2012.09.041) Copyright © 2013 Society for Vascular Surgery Terms and Conditions

Fig 3 The expression of matrix metalloproteinase (MMP)-2 and MMP-9 is increased by arterial shear stress (SS). A, MMP-2 transcripts are upregulated by arterial shear stress, and this upregulation is increased by high pressure (70 mm Hg). B, Western blots showed increased levels of both MMP-2 and MMP-9 after perfusion, under both low and high hemodynamic pressure. C and D, Both pro-MMP-2 and MMP-2 protein were significantly increased by perfusion and high pressure. E and F, MMP-9 transcript and protein levels were also upregulated by perfusion. However, the MMP-9 protein did not appear to be further induced by high pressure. Data represent mean ± standard error of the mean of two series of eight and nine experiments. GADPH, Glyceraldehyde 3-phosphate dehydrogenase. *P < .05; **P < .01; ***P < .001 vs nonperfused veins and veins exposed to low pressure. Journal of Vascular Surgery 2013 57, 1371-1382DOI: (10.1016/j.jvs.2012.09.041) Copyright © 2013 Society for Vascular Surgery Terms and Conditions

Fig 4 The lytic activity of matrix metalloproteinase (MMP)-2 and MMP-9 is increased by arterial shear stress (SS) and blood pressure. A, Zymographic analysis distinguished the gelatin lysis induced by pro-MMP-2, MMP-2, and MMP-9. B-D, Quantitative analysis showed that the activities of these enzymes increased in veins exposed to arterial shear stress. However, only MMP-2 and MMP-9 were further enhanced by high pressure. Data represent mean ± standard error of the mean of two series of eight and nine experiments. *P < .05; **P < .01; ***P < .001 vs control veins or veins submitted to low pressure. E, Immunostaining showed that both MMP-2 (top row) and MMP-9 (bottom row) increased mostly within the media layer of the perfused veins. L, Lumen; M, media. Bars represent 100 μm. Journal of Vascular Surgery 2013 57, 1371-1382DOI: (10.1016/j.jvs.2012.09.041) Copyright © 2013 Society for Vascular Surgery Terms and Conditions

Fig 5 The inhibitors of tissue inhibitor of metalloproteinase (TIMP)-1 and TIMP-2 are differentially affected by arterial shear stress (SS) and pressure. A and B, Veins exposed to arterial shear stress featured increased expression of TIMP-1 mRNA but decreased expression of the TIMP-2 transcripts. C, Consistent with the transcript change, Western blots showed upregulation of the TIMP-1 protein in the presence of both low and high pressure. D, In marked contrast to the mRNA changes, the levels of the TIMP-2 protein were markedly increased only in the presence of high pressure. Data represent mean ± standard error of the mean of two series of eight and nine experiments. *P < .05; **P < .01, ***P < .001 vs control veins. E, Immunostaining confirmed that the abundance of TIMP-1-positive and TIMP-2-positive smooth muscle cells increased under arterial hemodynamic conditions. GADPH, Glyceraldehyde 3-phosphate dehydrogenase; L, lumen; M, media; PAI, plasminogen activator inhibitor. Insets show the boxed areas at higher magnification. Bars represents 100 μm in figures and 400 μm in the insets. Journal of Vascular Surgery 2013 57, 1371-1382DOI: (10.1016/j.jvs.2012.09.041) Copyright © 2013 Society for Vascular Surgery Terms and Conditions

Fig 6 The levels of plasminogen activator inhibitor (PAI)-1 increase in human saphenous vein exposed to arterial shear stress (SS). A, PAI-1 transcripts were increased in human saphenous veins submitted to arterial shear stress. B and C, Western blot analysis and immunocytochemistry showed that PAI-1 mostly increased in the media layer of the arterializing veins. GADPH, Glyceraldehyde 3-phosphate dehydrogenase; L, lumen; M, media. Bar represents 50 μm. Data represent mean ± standard error of the mean of two series of eight and nine experiments. **P < .01; ***P < .001 vs control veins. Journal of Vascular Surgery 2013 57, 1371-1382DOI: (10.1016/j.jvs.2012.09.041) Copyright © 2013 Society for Vascular Surgery Terms and Conditions

Fig 7 The expression of Ephrin-B2 and Eph-B4 decreases in the presence of high pressure. A and B, The transcription of Ephrin-B2 and Eph-B4 decreased in veins submitted to arterial shear stress (SS). C and D, The levels of the cognate proteins were also reduced under these conditions. In vessels exposed to low pressure, only Eph-B4 was found significantly reduced. Data represent mean ± standard error of the mean of two series of eight and nine experiments. GADPH, Glyceraldehyde 3-phosphate dehydrogenase. **P < .01; ***P < .001 vs control veins. Journal of Vascular Surgery 2013 57, 1371-1382DOI: (10.1016/j.jvs.2012.09.041) Copyright © 2013 Society for Vascular Surgery Terms and Conditions