Tumor-Associated Neutrophils Show Phenotypic and Functional Divergence in Human Lung Cancer  Shilpi Saha, Subhra K. Biswas  Cancer Cell  Volume 30, Issue 1, Pages 11-13 (July 2016) DOI: 10.1016/j.ccell.2016.06.016 Copyright © 2016 Elsevier Inc. Terms and Conditions

Figure 1 Different Subsets of TANs in Human Lung Tumors Diagrammatic representation of the phenotypic and functional diversity of TANs in human lung tumors. Left: Early-stage tumors (<3 cm diameter) that are positive for IFNγ and GM-CSF show the presence of two distinct subsets of TANs: the APC-like hybrid TANs and the canonical TANs. Inset shows immature neutrophils in the presence of IFNγ and GM-CSF differentiating into hybrid TANs that co-express phenotypic markers of both canonical TANs (CD15, CD66b, CD11b) and APCs (HLA-DR, CD14, CD206, CD86, CCR7, HLA-ABC). These cells also possessed APC-like functional properties such as high phagocytic activity, LPS-induced inflammatory cytokine expression, and antigen-specific T cell responses (indicated by upward pointing arrows). Hypoxia limits differentiation of hybrid TANs. Right: Late-stage, larger tumors (5–7 cm in diameter) lack hybrid TANs and show infiltration of canonical TANs only. Although not shown in the study, larger tumors are often associated with tumor hypoxia (indicated by the yellow halo), which could possibly contribute to the lack of hybrid TAN differentiation in these tumors. Inset shows that canonical TANs phenotypically express only neutrophil markers and functionally show much lower ability to phagocytose, express inflammatory cytokines in response to LPS, and stimulate antigen-specific effector T cell responses (indicated by downward pointing arrows), as compared to the APC-like hybrid TANs. Cancer Cell 2016 30, 11-13DOI: (10.1016/j.ccell.2016.06.016) Copyright © 2016 Elsevier Inc. Terms and Conditions