Transfer of energy via ATP

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Transfer of energy via ATP

Higher Biology (January 2011) UNIT 2: Metabolism and Survival 1. Metabolism is essential for Life c) Cellular respiration (i) Transfer of energy via ATP Experiments on ATP dependent reactions, e.g. luciferase, luminescent reactions

Higher Human Biology (January 2011) UNIT 1: Human Cells 3. Cell metabolism b) Cellular respiration (i) Transfer of energy via ATP Experiments on ATP dependent reactions, e.g. luciferase, luminescent reactions

Luminescence Luminescence is emission of light by a substance not resulting from heat; it is thus a form of cold body radiation Source: http://en.wikipedia.org/wiki/Luminescence (accessed June 22nd 2012)

Bioluminescence

George McGavin Edith Widder

Flashlight fish Angler fish

Bioluminescence - Lifesaver?

Firefly bioluminescence: Emission oxidation of luciferin Proteins and substrates - mostly unrelated Most referred to as luciferin / luciferase.

Activity Two practicals: Cold Heat Adapt to the dark 10 min (approx) for each practical

Equipment / materials 2 Petri dishes Disposable pipettes Bijou / small bottle of ATP Bijou of buffer (on ice) Test-tube Water bath @ 60oC 1 (and only 1!) small bottle of firefly tails Red light