Volume 136, Issue 5, Pages e6 (May 2009)

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Volume 136, Issue 5, Pages 1796-1805.e6 (May 2009) A Polymorphism in MAPKAPK3 Affects Response to Interferon Therapy for Chronic Hepatitis C Hironobu Tsukada, Hidenori Ochi, Toshiro Maekawa, Hiromi Abe, Yoshifumi Fujimoto, Masataka Tsuge, Hiroshi Takahashi, Hiromitsu Kumada, Naoyuki Kamatani, Yusuke Nakamura, Kazuaki Chayama Gastroenterology Volume 136, Issue 5, Pages 1796-1805.e6 (May 2009) DOI: 10.1053/j.gastro.2009.01.061 Copyright © 2009 AGA Institute Terms and Conditions

Figure 1 Allele-specific transcript quantification of MAPKAPK3. The allele-specific MAPKAPK3 mRNA expression ratio for haplotype 2 to haplotype 1 is shown. Individual data from liver biopsies from 5 patients are indicated. Each experiment was performed in triplicate assay at least 3 times. Data represent the mean ± SD. Gastroenterology 2009 136, 1796-1805.e6DOI: (10.1053/j.gastro.2009.01.061) Copyright © 2009 AGA Institute Terms and Conditions

Figure 2 Effects of MAPKAPK3 on IFN-α–induced gene transcription via ISRE and GAS elements. Huh7 cells were transfected with both 1 ng renilla luciferase expression vector pRL-TK (internal control) and 10 ng firefly luciferase expression vector (A) pISRE-TA-Luc or (B) pGAS-TA-Luc, in conjunction with 40 ng expression plasmid pDEST51/mock (negative control, open bar), pDEST51/suppressor of cytokine signaling 1 (socs1) (positive control, black bar), or pDEST51/MAPKAPK3 (gray bar). After 24 hours, cells were stimulated with IFN-alfa for another 20 hours, and luciferase induction was measured. Firefly luciferase activity was normalized by renilla luciferase activity. Data represent the mean ± SD of triplicate assay. *P < .05 for comparison with mock. Gastroenterology 2009 136, 1796-1805.e6DOI: (10.1053/j.gastro.2009.01.061) Copyright © 2009 AGA Institute Terms and Conditions