M. A. McNulty, R. F. Loeser, C. Davey, M. F. Callahan, C. M

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Presentation transcript:

Histopathology of naturally occurring and surgically induced osteoarthritis in mice M.A. McNulty, R.F. Loeser, C. Davey, M.F. Callahan, C.M. Ferguson, C.S. Carlson Osteoarthritis and Cartilage Volume 20, Issue 8, Pages 949-956 (August 2012) DOI: 10.1016/j.joca.2012.05.001 Copyright © 2012 Osteoarthritis Research Society International Terms and Conditions

Fig. 1 Evaluation of CCD in the medial tibial plateaus from the DMM joints (means ± 95% CIs). (A) Mean percentage of CCD. ** = P < 0.0001 when DMM (n = 8) is compared to the Control joints (n = 11) of the 4.5 month old mice, ^^ = P < 0.0001 when compared DMM (n = 12) is compared to the Control joints (n = 15) of the 5.5 month old mice. (B) Mean Chondrocyte Viability factor scores. ** = P = 0.0003 when compared to the Control joints of the 4.5 month old mice, ^^ = P = 0.0002 when compared to the Control joints of the 5.5 month old mice. Osteoarthritis and Cartilage 2012 20, 949-956DOI: (10.1016/j.joca.2012.05.001) Copyright © 2012 Osteoarthritis Research Society International Terms and Conditions

Fig. 2 Histological sections demonstrating abaxial osteophytes located on the medial tibial plateaus of DMM joints. Bar = 100 μm. (A) Abaxial joint margin of the medial tibial plateau from Control joint (no osteophyte) from Study 1. (B) Abaxial osteophyte on the medial tibial plateau of a 4.5 month old DMM joint. Arrows indicate original abaxial joint margin. (C) Larger abaxial osteophyte on the medial tibial plateau of a 5.5 month old DMM joint. Arrows indicate original joint margin. Osteoarthritis and Cartilage 2012 20, 949-956DOI: (10.1016/j.joca.2012.05.001) Copyright © 2012 Osteoarthritis Research Society International Terms and Conditions

Fig. 3 Changes in AC and CCD in C57Bl/6 mice by age (means ± 95% CIs). (A) Mean percentage of CCD by age. * = 4.5 months (n = 8) compared to 5.5 months (P = 0.01, n = 15), 16 months (P = 0.05, n = 10), 17 months (P = 0.002, n = 16), and 23 months (P < 0.0001, n = 13); ^ = 5.5 months compared to 17 months (P = 0.02) and 23 months (P < 0.0001), and # = 17 months compared to 23 months (P = 0.03). (B) Mean AC thickness by age. * = 4.5 months when compared to 16 months (P = 0.018), 17 months (P = 0.005), and 23 months (P = 0.017). Osteoarthritis and Cartilage 2012 20, 949-956DOI: (10.1016/j.joca.2012.05.001) Copyright © 2012 Osteoarthritis Research Society International Terms and Conditions

Fig. 4 Histological sections demonstrating CCD identified in the mouse stifle joints and TUNEL staining. (A–B) Bar = 100 μm, (C–F) Bar = 50 μm; femoral condyle in top of images, tibial plateau on the bottom. Black arrows indicate areas of CCD, black arrow heads indicate tidemark, red arrows indicate TUNEL positive chondrocytes adjacent to areas of CCD. (A) 5.5mo DMM with a large area of CCD on the tibial plateau and femoral condyle not associated with significant cartilage degeneration. (B) 5.5mo DMM CCD in both the tibial plateau and femoral condyle, associated with extensive damage to the AC, including areas of full thickness loss. (C) 4.5mo DMM, TUNEL staining of section containing CCD in the tibial plateau. (D) 17mo joint with age-related OA, TUNEL staining of section containing CCD in the tibial plateau. (E) 5.5mo Control, TUNEL staining of section of tibial plateau with no cell death or OA and no positive staining. (F) Mouse thymus was used as a positive control. Osteoarthritis and Cartilage 2012 20, 949-956DOI: (10.1016/j.joca.2012.05.001) Copyright © 2012 Osteoarthritis Research Society International Terms and Conditions