Molecular Genetics Jeopardy Vocab DNA structure/ Replication Transcription Translation Mutations $100 $100 $100 $100 $100 $200 $200 $200 $200 $200 $300 $300 $300 $300 $300 $400 $400 $400 $400 $400 $500 $500 $500 $500 $500 Final Jeopardy

1 - $100 What happens in transcription and translation? Transcription: mRNA molecule made from DNA Translation: amino acid sequence made from mRNA

1 - $200 What enzyme adds nucleotides to a growing piece of DNA during DNA replication? DNA polymerase

1 - $300 What are the three types of RNA mRNA, tRNA, rRNA

1 - $400 What are introns and exons? During mRNA processing, introns are cut out and exons stay in

1 - $500 What is this called (circled) and what are each of the parts called Nucleotide-made up of sugar, phosphate and nitrogenous base

2 - $100 What are the bases that make up DNA? Which pair with which? Adenine-thymine, cytosine-guanine

2 - $200 What protein unwinds DNA during replication? helicase

2 - $300 If one strand of DNA reads: 3’ ATGCGTA 5’ what will the other strand read? 5’ TACGCAT 3’

2 - $400 Why is DNA replication called semi-conservative?

2 - $500 Label the parts of DNA replication. A-helicase B-leading strand C-lagging strand D-DNA polymerase E-5’ F-3’ B C D

3 - $100 What is the product of transcription? mRNA molecule

3 - $200 If the DNA sequence of a gene begins with ATGCTG, what will the mRNA read after transcription? UACGAC

3 - $300 Where does transcription occur and why? In the nucleus because DNA is in the nucleus

3 - $400 What are three differences between DNA and RNA? DNA is double stranded, has deoxyribose sugar, has T instead of U

3 - $500 What happens during mRNA processing? Introns spliced out, 5’ cap and poly A tail

4 - $100 Where does translation occur? ribosome

4 - $200 What is this molecule called? tRNA

4 - $300 What are codons and anticodons? Codons=3 base pairs of mRNA that code for an amino acid anticodon-=complementary sequence on tRNA molecule

4 - $400 If an mRNA sequence reads AUGGAU, what will the anti codons of the tRNA molecules that bind to the mRNA be? UAC, CUA

4 - $500 If an mRNA sequence reads AUG CCA GUG, what will the sequence of amino acids be after translation? Met, pro, val

5 - $100 What type of mutation is this? Normal: ACGAGG Mutated: CGAGG Deletion, frameshift

5 - $200 What type of mutation is this? Normal: AGTGTA Mutated: AGTGATA Insertion, frameshift

5 - $300 Why is the difference between a frameshift and a substitution mutation? A frameshift changes all amino acids after the mutation

5 - $400 What is it called if a mutation leads to a codon changing to a stop codon? nonsense

5 - $500 What is the difference between missense, nonsense and silent mutations? Missense-changes one amino acid Nonsense-changes to a stop codon Silent-doesn’t change amino acid

Final Jeopardy Given the following template strand DNA sequence, what will the mRNA produced from transcription be? What will the amino acid sequence after translation be? TACGGAACGCTG