Antagonistic effect of the inflammasome on thymic stromal lymphopoietin expression in the skin Sonia Schuepbach-Mallepell, PhD, Virginie Philippe, PhD,

Slides:

Advertisements

Similar presentations

TNF-like weak inducer of apoptosis (TWEAK) and TNF-α cooperate in the induction of keratinocyte apoptosis Maya Zimmermann, PhD, Andrea Koreck, MD, Norbert.

Advertisements

Multiple-checkpoint inhibition of thymic stromal lymphopoietin–induced TH2 response by TH17-related cytokines Sofia I. Bogiatzi, BSc, Maude Guillot-Delost,

Toll-like receptor 2 ligands promote chronic atopic dermatitis through IL-4–mediated suppression of IL-10 Susanne Kaesler, PhD, Thomas Volz, MD, Yuliya.

Signal transducer and activator of transcription 1 decoy oligodeoxynucleotide suppression of contact hypersensitivity Andreas H. Wagner, PhD, Inka Wittjen,

Natalija Novak, MD Journal of Allergy and Clinical Immunology

Russell S. Traister, MD, PhD, Crystal E. Uvalle, BS, Gregory A

Endotype-driven care pathways in patients with chronic rhinosinusitis

Platelets play important roles in the late phase of the immediate hypersensitivity reaction Risa Tamagawa-Mineoka, MD, PhD, Norito Katoh, MD, PhD, Saburo.

Mechanical injury polarizes skin dendritic cells to elicit a TH2 response by inducing cutaneous thymic stromal lymphopoietin expression Michiko K. Oyoshi,

IL-4 induces IL-13–independent allergic airway inflammation

IL-17–producing T cells in lung immunity and inflammation

Thymic stromal lymphopoietin downregulates filaggrin expression by signal transducer and activator of transcription 3 (STAT3) and extracellular signal-regulated.

The role of air pollutants in atopic dermatitis

Prostaglandin E2 suppresses CCL27 production through EP2 and EP3 receptors in human keratinocytes Naoko Kanda, MD, PhD, Hiroshi Mitsui, MD, PhD, Shinichi.

Cigarette smoke extract induces thymic stromal lymphopoietin expression, leading to TH2-type immune responses and airway inflammation Yuki Nakamura,

Epicutaneous sensitization results in IgE-dependent intestinal mast cell expansion and food-induced anaphylaxis Lisa M. Bartnikas, MD, Michael F. Gurish,

Autocrine hemokinin-1 functions as an endogenous adjuvant for IgE-mediated mast cell inflammatory responses Tina L. Sumpter, PhD, Chin H. Ho, MD, Anna.

Cytokine milieu modulates release of thymic stromal lymphopoietin from human keratinocytes stimulated with double-stranded RNA Hirokazu Kinoshita, MD,

Is 9 more than 2 also in allergic airway inflammation?

Responsiveness to respiratory syncytial virus in neonates is mediated through thymic stromal lymphopoietin and OX40 ligand Junyan Han, PhD, Azzeddine.

Toll-like receptor 9 suppression in plasmacytoid dendritic cells after IgE-dependent activation is mediated by autocrine TNF-α John T. Schroeder, PhD,

The IL-17F signaling pathway is involved in the induction of IFN-γ–inducible protein 10 in bronchial epithelial cells Mio Kawaguchi, MD, Fumio Kokubu,

TNF-like weak inducer of apoptosis (TWEAK) and TNF-α cooperate in the induction of keratinocyte apoptosis Maya Zimmermann, PhD, Andrea Koreck, MD, Norbert.

Toll-like receptor 3 enhances late-phase reaction of experimental allergic conjunctivitis Mayumi Ueta, MD, PhD, Satoshi Uematsu, MD, PhD, Shizuo Akira,

Specific epicutaneous immunotherapy prevents sensitization to new allergens in a murine model Lucie Mondoulet, PhD, Vincent Dioszeghy, PhD, Emilie Puteaux,

Critical role of IgE-dependent mast cell activation in a murine model of allergic conjunctivitis Ken Fukuda, MD, PhD, Masaharu Ohbayashi, PhD, Kei Morohoshi,

Thymic stromal lymphopoietin activation of basophils in patients with allergic asthma is IL-3 dependent Brittany M. Salter, BSc, John Paul Oliveria,

Dexamethasone regulation of thymic stromal lymphopoietin receptor expression on mast cells and their precursors Zoulfia Allakhverdi, PhD, Michael R.

Exposure to food allergens through inflamed skin promotes intestinal food allergy through the thymic stromal lymphopoietin–basophil axis Mario Noti,

Bcl2-like protein 12 plays a critical role in development of airway allergy through inducing aberrant TH2 polarization Zhi-Qiang Liu, MD, PhD, Ying Feng,

Thymic stromal lymphopoietin converts human epidermal Langerhans cells into antigen- presenting cells that induce proallergic T cells Susanne Ebner, PhD,

Davide Pennino, MSc, Pankaj K

Exaggerated IL-17 response to epicutaneous sensitization mediates airway inflammation in the absence of IL-4 and IL-13 Rui He, MD, PhD, Hye Young Kim,

Multiple-checkpoint inhibition of thymic stromal lymphopoietin–induced TH2 response by TH17-related cytokines Sofia I. Bogiatzi, BSc, Maude Guillot-Delost,

Antiviral activity of human β-defensin 3 against vaccinia virus

IL-5 T-cell responses to house dust mite are associated with the development of allergen-specific IgE responses and asthma in the first 5 years of life

Toll-like receptor 2 is important for the TH1 response to cutaneous sensitization Haoli Jin, MD, PhD, Lalit Kumar, PhD, Clinton Mathias, PhD, David Zurakowski,

Thymic stromal lymphopoietin–activated invariant natural killer T cells trigger an innate allergic immune response in atopic dermatitis Wen Hao Wu, PhD,

IL-17 in atopic eczema: Linking allergen-specific adaptive and microbial-triggered innate immune response Kilian Eyerich, MD, Davide Pennino, PhD, Claudia.

Does reduced zona pellucida binding protein 2 (ZPBP2) expression on chromosome 17q21 protect against asthma? Marina Miller, MD, PhD, Christine Vuong,

Receptor-interacting protein kinase 3 controls keratinocyte activation in a necroptosis- independent manner and promotes psoriatic dermatitis in mice

Allergic skin sensitization promotes eosinophilic esophagitis through the IL-33–basophil axis in mice Nicholas Venturelli, BS, Willem S. Lexmond, MD,

IL-12 affects Dermatophagoides farinae–induced IL-4 production by T cells from pediatric patients with mite-sensitive asthma Takeshi Noma, MD, PhD, Izumi.

Novel allergic asthma model demonstrates ST2-dependent dendritic cell targeting by cypress pollen Lucia Gabriele, BS, Giovanna Schiavoni, BS, Fabrizio.

Langerhans cells are critical in epicutaneous sensitization with protein antigen via thymic stromal lymphopoietin receptor signaling Saeko Nakajima,

Autophagy: Nobel Prize 2016 and allergy and asthma research

IL-17E upregulates the expression of proinflammatory cytokines in lung fibroblasts Séverine Létuvé, PhD, Stéphane Lajoie-Kadoch, MSc, Séverine Audusseau,

Mast cells regulate IFN-γ expression in the skin and circulating IgE levels in allergen- induced skin inflammation Harri Alenius, PhD, Dhafer Laouini,

Chemokine responses distinguish chemical-induced allergic from irritant skin inflammation: Memory T cells make the difference Stephan Meller, MD, Antti.

Correlation between CCL26 production by human bronchial epithelial cells and airway eosinophils: Involvement in patients with severe eosinophilic asthma

Toll-like receptor 2 ligands promote chronic atopic dermatitis through IL-4–mediated suppression of IL-10 Susanne Kaesler, PhD, Thomas Volz, MD, Yuliya.

Short ragweed pollen triggers allergic inflammation through Toll-like receptor 4– dependent thymic stromal lymphopoietin/OX40 ligand/OX40 signaling pathways

The steroidogenic enzyme Cyp11a1 is essential for development of peanut-induced intestinal anaphylaxis Meiqin Wang, MD, PhD, Julita Ramirez, DVM, PhD,

Physiologic assessment of allergic rhinitis in mice: Role of the high-affinity IgE receptor (FcɛRI) Satoko Miyahara, MD, PhD, Nobuaki Miyahara, MD, PhD,

Eosinophilic esophagitis treated with immunotherapy to dust mites

IL-25 enhances allergic airway inflammation by amplifying a TH2 cell–dependent pathway in mice Tomohiro Tamachi, MD, Yuko Maezawa, MD, PhD, Kei Ikeda,

IgE induces transcriptional regulation of thymic stromal lymphopoietin in human airway smooth muscle cells Naresh Singh Redhu, MSc, Ali Saleh, PhD, Hai-Chon.

Does “autoreactivity” play a role in atopic dermatitis?

Thymic stromal lymphopoietin and OX40 ligand pathway in the initiation of dendritic cell–mediated allergic inflammation Yong-Jun Liu, MD, PhD Journal.

IL-22 attenuates IL-25 production by lung epithelial cells and inhibits antigen-induced eosinophilic airway inflammation Kentaro Takahashi, MD, Koichi.

Zhenying Nie, PhD, Cole S. Nelson, PhD, David B. Jacoby, MD, Allison D

IL-9 and c-Kit+ mast cells in allergic rhinitis during seasonal allergen exposure: Effect of immunotherapy Kayhan T. Nouri-Aria, PhD, FRCPath, Charles.

Ovalbumin-specific IgE modulates ovalbumin-specific T-cell response after repetitive oral antigen administration Nemuko Omata, MD, Yusei Ohshima, MD,

Prostaglandin E2–EP3 signaling suppresses skin inflammation in murine contact hypersensitivity Tetsuya Honda, MD, PhD, Toshiyuki Matsuoka, MD, PhD, Mayumi.

Cytokine modulation of atopic dermatitis filaggrin skin expression

Epicutaneous immunization with ovalbumin and CpG induces TH1/TH17 cytokines, which regulate IgE and IgG2a production Monika Majewska-Szczepanik, PhD,

Nrf2 activation by sulforaphane restores the age-related decrease of TH1 immunity: Role of dendritic cells Hyon-Jeen Kim, PhD, Berenice Barajas, BS,

Systemic responses after bronchial aspirin challenge in sensitive patients with asthma Joanna S. Makowska, MD, PhD, Janina Grzegorczyk, PhD, Barbara Bienkiewicz,

IL-31 is associated with cutaneous lymphocyte antigen–positive skin homing T cells in patients with atopic dermatitis Janine Bilsborough, PhD, Donald.

Natural history of cow’s milk allergy

Presentation transcript:

Antagonistic effect of the inflammasome on thymic stromal lymphopoietin expression in the skin Sonia Schuepbach-Mallepell, PhD, Virginie Philippe, PhD, Marie-Charlotte Brüggen, MD, Hideki Watanabe, PhD, Stéphanie Roques, BSc, Christine Baldeschi, PhD, Olivier Gaide, MD, PhD Journal of Allergy and Clinical Immunology Volume 132, Issue 6, Pages 1348-1357 (December 2013) DOI: 10.1016/j.jaci.2013.06.033 Copyright © 2013 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 1 The TH2 response is not affected by ASC or IL-1R deficiency in 2 AD models. A, Experimental scheme for Fig 1, B through E. B, Stronger response of BALB/c than C57Bl/6 mice to aDBP-FITC–induced AD. C, Response to aDBP-FITC in ASC- or IL-1R–deficient mice (n = 9, 3 independent experiments). D, aDBP-FITC–induced cytokine pattern (high IL-4/low IFN-γ = TH2 biased) in ASC- and IL-1R–deficient mice (n = 6, 2 independent experiments). E, T cell– and eosinophil-dominated skin infiltration after aDBP-FITC (n = 4). Bar = 0.2 mm. F, Ear swelling and IgE class-switching in a model of AD induced by DNCB (n = 9, 3 independent experiments). Error bars = SDs. Journal of Allergy and Clinical Immunology 2013 132, 1348-1357DOI: (10.1016/j.jaci.2013.06.033) Copyright © 2013 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 2 DBP upregulates TSLP independently of the inflammasome, and both DNFB and IL-1β antagonize its induction. A, Experimental scheme for Fig 2, B through G. B, Real-time qPCR for mTSLP (n = 6, 2 independent experiments). C, TSLP levels in C57BL/6 versus BALB/c mice in response to aDBP. D, TSLP levels after aDBP stimulation in IL-1R– or ASC-deficient mice. E, Effect of IL-1β injection on TSLP mRNA levels (n = 6, 2 independent experiments). F, Effect of DNFB on basal and DBP-induced TSLP mRNA levels (n = 6, 2 independent experiments). G, Comparative TSLP mRNA levels in the epidermis and dermis of dispase-split ear skin (n = 3). H, TSLP mRNA levels in human skin xenotransplants (n = 2). Error bars = SDs. I, TSLP mRNA levels in human skin equivalents (epidermis only; n = 4). Error bars = SDs. J, TSLP protein expression in normal and AD human skin biopsy specimens at ×10 and ×40 magnification. Bars = 50 μm. Journal of Allergy and Clinical Immunology 2013 132, 1348-1357DOI: (10.1016/j.jaci.2013.06.033) Copyright © 2013 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 3 IL-1 plays a key role in the negative regulation of TSLP by the inflammasome. TSLP mRNA levels 24 hours after 3 exposures to aDBP, DNFB, or both are shown. A, BALB/c mice: effect of the presence of IL-1R on TSLP levels. B, C57BL/6 mice: effect of the overexpression of transgenic IL-Ra on TSLP levels. C, BALB/C mice: effect of the intraperitoneal injection of recombinant IL-1Ra on TSLP levels. Error bars = SDs. Journal of Allergy and Clinical Immunology 2013 132, 1348-1357DOI: (10.1016/j.jaci.2013.06.033) Copyright © 2013 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 4 Negative regulation of TSLP expression by the inflammasome in cultured keratinocytes. TSLP mRNA levels after DBP, 9-cis-RA, tumor necrosis factor, IL-1β, or DNFB exposure. A, PAM212 murine keratinocytes. B, N-terts and HaCaT human keratinocytes. C, Primary murine keratinocytes. D, Primary human keratinocytes. E, MLE12 murine pneumocytes. F, A549 human pneumocytes. *P < .05 and **P < .01. Error bars = SDs. All experiments were done in triplicate in 3 or more independent experiments. Journal of Allergy and Clinical Immunology 2013 132, 1348-1357DOI: (10.1016/j.jaci.2013.06.033) Copyright © 2013 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 5 DBP can redirect the DNTB response toward a TH2 response in mice. A, Ear swelling 24 or 48 hours after challenge of sensitized mice. B, RNA levels 24 hours after challenge showing the presence of T cells of the TH2 type (high IL-4/low IFN-γ) or TH1 type (opposite pattern). C, Skin peroxidase activity 24 hours after challenge as a measure of eosinophil infiltration (n = 6 in 2 independent experiments). Error bars = SDs. Journal of Allergy and Clinical Immunology 2013 132, 1348-1357DOI: (10.1016/j.jaci.2013.06.033) Copyright © 2013 American Academy of Allergy, Asthma & Immunology Terms and Conditions