A Cryotransmission Electron Microscopy Study of Skin Barrier Formation

Slides:

Advertisements

Similar presentations

Persistence of Both Peripheral and Non-Peripheral Corneodesmosomes in the Upper Stratum Corneum of Winter Xerosis Skin Versus only Peripheral in Normal.

Advertisements

Localization of Ceramide and Glucosylceramide in Human Epidermis by Immunogold Electron Microscopy Gabriele Vielhaber, Stephan Pfeiffer, Lore Brade,

Negative Electric Potential Induces Alteration of Ion Gradient and Lamellar Body Secretion in the Epidermis, and Accelerates Skin Barrier Recovery After.

The Extent of Orthorhombic Lipid Phases in the Stratum Corneum Determines the Barrier Efficiency of Human Skin In Vivo Fabienne Damien, Mila Boncheva

Melanocytes Derived from Patients with Hermansky–Pudlak Syndrome Types 1, 2, and 3 Have Distinct Defects in Cargo Trafficking Bonnie Richmond, Marjan.

Joke A. Bouwstra, Anko de Graaff, Gert S. Gooris

Vitamin C Regulates Keratinocyte Viability, Epidermal Barrier, and Basement Membrane In Vitro, and Reduces Wound Contraction After Grafting of Cultured.

Stratum Corneum Keratin Structure, Function, and Formation: The Cubic Rod-Packing and Membrane Templating Model Lars Norlén, Ashraf Al-Amoudi Journal.

Epidermal Lamellar Granules Transport Different Cargoes as Distinct Aggregates Akemi Ishida-Yamamoto, Michel Simon, Mari Kishibe, Yuki Miyauchi, Hidetoshi.

Stratum Corneum Keratin Structure, Function, and Formation: The Cubic Rod-Packing and Membrane Templating Model Lars Norlén, Ashraf Al-Amoudi Journal.

Skin Barrier Formation: The Membrane Folding Model

Penetration Pathways Induced by Low-Frequency Sonophoresis with Physical and Chemical Enhancers: Iron Oxide Nanoparticles versus Lanthanum Nitrates Sang.

Omega-Hydroxyceramides are Required for Corneocyte Lipid Envelope (CLE) Formation and Normal Epidermal Permeability Barrier Function Martin Behne, Yoshikazu.

25 Hydroxyvitamin D 1 α-Hydroxylase Is Required for Optimal Epidermal Differentiation and Permeability Barrier Homeostasis D.D. Bikle, S. Chang, D. Crumrine,

Christina A. Young, Richard L

Calcium Ion Gradients and Dynamics in Cultured Skin Slices of Rat Hindpaw in Response to Stimulation with ATP Moe Tsutsumi, Sumiko Denda, Kaori Inoue,

Nanostructure of Cationic Lipid-Oligonucleotide Complexes

Anna Celli, Debra Crumrine, Jason M. Meyer, Theodora M. Mauro

Shao Jun Jiang, Sang Min Hwang, Eung Ho Choi, Sung Ku Ahn

Large-Scale Electron Microscopy Maps of Patient Skin and Mucosa Provide Insight into Pathogenesis of Blistering Diseases Ena Sokol, Duco Kramer, Gilles.

Sensitivity and Specificity of Multiphoton Laser Tomography for In Vivo and Ex Vivo Diagnosis of Malignant Melanoma Enrico Dimitrow, Mirjana Ziemer,

Matthias Schmuth, Gil Yosipovitch, Mary L

Normalization of Epidermal Calcium Distribution Profile in Reconstructed Human Epidermis Is Related to Improvement of Terminal Differentiation and Stratum.

Visible Radiation Affects Epidermal Permeability Barrier Recovery: Selective Effects of Red and Blue Light Mitsuhiro Denda, Shigeyoshi Fuziwara Journal.

Doris A. Schwindt, Klaus P. Wilhelm, Howard I. Maibach

Origin of the Corneocyte Lipid Envelope (CLE): Observations in Harlequin Ichthyosis and Cultured Human Keratinocytes Peter M. Elias, Manigé Fartasch,

Hydration Disrupts Human Stratum Corneum Ultrastructure

Quasi-Normal Cornified Cell Envelopes in Loricrin Knockout Mice Imply the Existence of a Loricrin Backup System Michal Jarnik, Pierre A. de Viragh, Elisabeth.

Nanostructure of the Epidermal Extracellular Space as Observed by Cryo-Electron Microscopy of Vitreous Sections of Human Skin Ashraf Al-Amoudi, Jacques.

Establishment of Two Mouse Models for CEDNIK Syndrome Reveals the Pivotal Role of SNAP29 in Epidermal Differentiation Stina A. Schiller, Christina Seebode,

Low-Frequency Sonophoresis: Ultrastructural Basis for Stratum Corneum Permeability Assessed Using Quantum Dots Sumit Paliwal, Gopinathan K. Menon, Samir.

Changing Pattern of Deiminated Proteins in Developing Human Epidermis

View of Normal Human Skin In Vivo as Observed Using Fluorescent Fiber-Optic Confocal Microscopic Imaging Lucinda D. Swindle, Steven G. Thomas, Michael.

Marked Changes in Lamellar Granule and Trans-Golgi Network Structure Occur during Epidermal Keratinocyte Differentiation Haruyo Yamanishi, Tsutomu Soma,

Water Distribution and Natural Moisturizer Factor Content in Human Skin Equivalents Are Regulated by Environmental Relative Humidity Joke A. Bouwstra,

Exposure to a Dry Environment Enhances Epidermal Permeability Barrier Function Mitsuhiro Denda, Junko Sato, Yoshiko Masuda, Toru Tsuchiya, Junichi Koyama,

Volume 74, Issue 5, Pages (May 1998)

Direct Visualization of Lipid Domains in Human Skin Stratum Corneum's Lipid Membranes: Effect of pH and Temperature I. Plasencia, L. Norlén, L.A. Bagatolli

The “Caveolae Brake Hypothesis” and the Epidermal Barrier

Francois le Pelletier, Anne Janin Journal of Investigative Dermatology

Junko Sato, Mitsuhiro Denda, PhD, Sandra Chang, Peter M

Human Glands of Moll: Histochemical and Ultrastructural Characterization of the Glands of Moll in the Human Eyelid Beate M. Stoeckelhuber Journal of.

Barrier Characteristics of Different Human Skin Types Investigated with X-Ray Diffraction, Lipid Analysis, and Electron Microscopy Imaging Volker Schreiner,

Cellular Changes that Accompany Shedding of Human Corneocytes

Role of Peroxisome Proliferator-Activated Receptor α in Epidermal Development in Utero Matthias Schmuth, M.D., V.A., Kristina Schoonjans, Qian-Chun Yu,

Basis For Abnormal Desquamation And Permeability Barrier Dysfunction in RXLI Peter M. Elias, Debra Crumrine, Ulrich Rassner, Jean-Pierre Hachem, Gopinathan.

Epidermal Organization and Differentiation of HaCaT Keratinocytes in Organotypic Coculture with Human Dermal Fibroblasts Veronika M. Schoop, Norbert.

Skin Barrier Structure and Function: The Single Gel Phase Model

Electron Diffraction Provides New Information on Human Stratum Corneum Lipid Organization Studied in Relation to Depth and Temperature Gonneke S.K. Pilgram,

Periderm Cells Form Cornified Cell Envelope in Their Regression Process During Human Epidermal Development Masashi Akiyama Journal of Investigative.

Abnormal Cornified Cell Envelope Formation in Mutilating Palmoplantar Keratoderma Unrelated to Epidermal Differentiation Complex Masashi Akiyama, Angela.

Barrier Dysfunction and Pathogenesis of Neutral Lipid Storage Disease with Ichthyosis (Chanarin–Dorfman Syndrome) Marianne Demerjian, Debra A. Crumrine,

The Effects of Depilatory Agents as Penetration Enhancers on Human Stratum Corneum Structures Jin-Ning Lee, Shiou-Hwa Jee, Chih-Chieh Chan, Wen Lo, Chen-Yuan.

Hydration Disrupts Human Stratum Corneum Ultrastructure

Salvador González, Robert Sackstein, R

William L. Pickens, Ronald R. Warner, Ying L. Boissy, Raymond E

Re-epithelialization of Porcine Skin By The Sweat Apparatus

Rab11 Is Associated with Epidermal Lamellar Granules

Reconstruction of a Human Skin Equivalent Using a Spontaneously Transformed Keratinocyte Cell Line (HaCaT) Esther Boelsma, Mary C.H. Verhoeven, Maria.

Elafin, a Secretory Protein, is Cross-Linked into the Cornified Cell Envelopes from the Inside of Psoriatic Keratinocytes Hiroshi Nakane, Akemi Ishida-Yamamoto,

Activation of TLR3 in Keratinocytes Increases Expression of Genes Involved in Formation of the Epidermis, Lipid Accumulation, and Epidermal Organelles

Alterations in Desmosome Size and Number Coincide with the Loss of Keratinocyte Cohesion in Skin with Homozygous and Heterozygous Defects in the Desmosomal.

Journal of Investigative Dermatology

Truncation of CGI-58 Protein Causes Malformation of Lamellar Granules Resulting in Ichthyosis in Dorfman-Chanarin Syndrome Masashi Akiyama, Daisuke Sawamura,

Gopinathan K. Menon Journal of Investigative Dermatology

Research Techniques Made Simple: Drug Delivery Techniques, Part 1: Concepts in Transepidermal Penetration and Absorption Shawn Schmieder, Parth Patel,

Novel ALDH3A2 Heterozygous Mutations Are Associated with Defective Lamellar Granule Formation in a Japanese Family of Sjögren–Larsson Syndrome Akihiko.

Barrier Function of the Skin: “La Raison d'Être” of the Epidermis

Localization of Ceramide and Glucosylceramide in Human Epidermis by Immunogold Electron Microscopy Gabriele Vielhaber, Stephan Pfeiffer, Lore Brade,

Human Glands of Moll: Histochemical and Ultrastructural Characterization of the Glands of Moll in the Human Eyelid Beate M. Stoeckelhuber Journal of.

Presentation transcript:

A Cryotransmission Electron Microscopy Study of Skin Barrier Formation Lars Norlén Journal of Investigative Dermatology Volume 120, Issue 4, Pages 555-560 (April 2003) DOI: 10.1046/j.1523-1747.2003.12102.x Copyright © 2003 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 Apparent active sites of skin barrier formation (open white arrows) are distributed all around the border of the stratum granulosum/stratum corneum transition cell. Cryotransmission electron micrograph of the stratum granulosum/stratum corneum interface. Defocus: –6μm. Optical resolution: 5nm. Scale bar 500nm. Journal of Investigative Dermatology 2003 120, 555-560DOI: (10.1046/j.1523-1747.2003.12102.x) Copyright © 2003 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 2 (a) A new “organelle or branched tubular structure” (open white arrows), corresponding to “lamellar bodies” of classical chemical fixation electron micrographs, and a new “ribosome complex-like structure” (open black arrows), not preserved in classical electron micrographs, were both omnipresent at apparent active sites of skin barrier formation. Cryotransmission electron micrograph of the stratum granulosum/stratum corneum interface. T: transition cell; SC1: lowermost cornified cell. Black and white asterix: multifolded or multivesicular membrane complex. TF: tonofilaments. Defocus: –15μm. Optical resolution: 8nm. Scale bar: 200nm. (b) Classical chemical fixation electron micrographs of the stratum granulosum/stratum corneum interface characteristically shows large artifactual “empty” areas (black asterix) and artifactual aggregation of tonofilament structures (TF) into distinct bundles. MG: lamellar body within stratum granulosum cell; EMG: lamellar body in the intercellular space; CE: cornified envelope. With permission from the Macmillan Press Ltd (Stenn 1983, p 577). Journal of Investigative Dermatology 2003 120, 555-560DOI: (10.1046/j.1523-1747.2003.12102.x) Copyright © 2003 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 3 The new “organelles or branched tubular structures” contain granular material (open black arrow) with or without circular (open white arrow) or tubular-like (left inset) or multilamellar (upper right inset) inclusions. Cryotransmission electron micrographs of the stratum granulosum/stratum corneum interface. Note the similarities between the partly granular/lamellar content of the “organelles or branched tubular structure” (right upper inset) and the cubic-to-lamellar membrane transition (right lower inset), i.e., foldings/unfoldings between cubic (three-dimensional hyperbolic) and lamellar (two-dimensionally Euclidian) membrane structures, in a classical electron micrograph of mitochondriae of cardiac muscle cells of Serinus canarius (canary) (Slautterback, 1965;Landh, 1996, p 81, with permission). Cubic membranes can, in fact, sometimes be unambiguously identified and defined from two-dimensional electron micrographs through a direct template-correlation procedure (Landh, 1996, pp 67–69). T: transition cell; SC1: lowermost cornified cell; solid black arrow: membrane complex; solid white arrow: invagination of the transition cell plasma membrane. Defocus: –13μm. Optical resolution: 7nm. Scale bar: 100nm. Journal of Investigative Dermatology 2003 120, 555-560DOI: (10.1046/j.1523-1747.2003.12102.x) Copyright © 2003 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 4 The new “ribosome complex-like structure” is derived from a large electron lucent structure with a “multifolded” appearance. Cryotransmission electron micrograph of a new “ribosome complex-like structure”, not preserved in classical electron micrographs, at the stratum granulosum/transition cell interface. Note the seemingly continuous transition (white asterix) between a large electron lucent structure with a “multifolded” appearance (trans-Golgi complex?) (open white arrow) and the new “ribosome complex-like structure” (open black arrows), and its resemblance to the cubic-to-cubic membrane transition in a classical electron micrograph of a single and coherent endoplasmic reticulum (ER) of differentiating sieve elements (inset, upper half: di-gyroid ER with a lattice parameter of 140nm; inset, lower half: mono-gyroid ER with a lattice parameter of 50nm) (with permission:Behnke, 1968;Landh, 1996). SG: uppermost nucleated stratum granulosum cell; T: transition cell; D: desmosome; black asterix: invagination of the plasma membrane. Defocus: –13μm. Optical resolution: 7nm. Scale bar: 100nm. Journal of Investigative Dermatology 2003 120, 555-560DOI: (10.1046/j.1523-1747.2003.12102.x) Copyright © 2003 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 5 A new “continuous multilamellar structure”, which may correspond to the “extensive tubuloreticular cisternal membrane system” reported byElias et al (1998), was identified. Cryotransmission electron micrograph of a new “continuous multilamellar structure” close to the transition cell/stratum corneum interface. Note the similar layered OD profile of a region of the intercellular space (solid black arrow) at an active site of skin barrier formation, and of new “continuous multilamellar structure” (solid white arrow). Note further the strongly folded multilamellar appearance of the intercellular space (open black arrow). Inset: cryo-electron micrograph of a similar folded multilamellar continuous structure in conjunction with a new “organelle or branched tubular structure” with circular inclusion (Fig 3, open white arrows). Black asterix: bubbling artifact; T: transition cell; SC1: lowermost cornified cell. Defocus: –9μm. Optical resolution: 6nm. Scale bar: 100nm. Journal of Investigative Dermatology 2003 120, 555-560DOI: (10.1046/j.1523-1747.2003.12102.x) Copyright © 2003 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 6 The stratum corneum intercellular multilamellar lipid matrix is clearly visualized by cryotransmission electron microscopy. Cryotransmission electron micrograph of the intercellular space at the stratum granulosum/stratum corneum interface. Median lamellar repeat distance 6.0nm (range 5.5–6.5nm, n=10). T: transition cell; SC1: lowermost stratum corneum cell. Defocus: –5μm. Optical resolution: 3nm. Scale bar: 100nm. Journal of Investigative Dermatology 2003 120, 555-560DOI: (10.1046/j.1523-1747.2003.12102.x) Copyright © 2003 The Society for Investigative Dermatology, Inc Terms and Conditions