Development and Laboratory Evaluation of a Real-Time PCR Assay for Detecting Viruses and Bacteria of Relevance for Community-Acquired Pneumonia  Alicia.

Slides:



Advertisements
Similar presentations
Measurement of Relative Copy Number of CDKN2A/ARF and CDKN2B in Bladder Cancer by Real-Time Quantitative PCR and Multiplex Ligation-Dependent Probe Amplification.
Advertisements

Development of a Novel One-Tube Isothermal Reverse Transcription Thermophilic Helicase-Dependent Amplification Platform for Rapid RNA Detection James Goldmeyer,
CyclinD1/CyclinD3 Ratio by Real-Time PCR Improves Specificity for the Diagnosis of Mantle Cell Lymphoma Carol D. Jones, Katherine H. Darnell, Roger A.
Quantification of Parvovirus B19 DNA Using COBAS AmpliPrep Automated Sample Preparation and LightCycler Real-Time PCR Stefan Schorling, Gunnar Schalasta,
Design and Multiseries Validation of a Web-Based Gene Expression Assay for Predicting Breast Cancer Recurrence and Patient Survival Ryan K. Van Laar The.
KRAS and BRAF Mutation Analysis in Routine Molecular Diagnostics
P.-Y. Lévy  Clinical Microbiology and Infection 
Ran Zhuo, Brendon D. Parsons, Bonita E. Lee, Steven J
KRAS and BRAF Mutation Analysis in Routine Molecular Diagnostics
Performance Characteristics of a Quantitative Hepatitis C Virus RNA Assay Using COBAS AmpliPrep Total Nucleic Acid Isolation and COBAS TaqMan Hepatitis.
Real-Time Polymerase Chain Reaction for Detecting Bacterial DNA Directly from Blood of Neonates Being Evaluated for Sepsis  Jeanne A. Jordan, Mary Beth.
Real-Time PCR for Chlamydia pneumoniae Utilizing the Roche Lightcycler and a 16S rRNA Gene Target  Justin Hardick, Nancy Maldeis, Mellisa Theodore, Billie.
Laboratory Guidelines for Detection, Interpretation, and Reporting of Maternal Cell Contamination in Prenatal Analyses  Narasimhan Nagan, Nicole E. Faulkner,
Development and Evaluation of a SYBR Green–Based Real-Time Multiplex RT-PCR Assay for Simultaneous Detection and Serotyping of Dengue and Chikungunya.
A DNA Real-Time Quantitative PCR Method Suitable for Routine Monitoring of Low Levels of Minimal Residual Disease in Chronic Myeloid Leukemia  Paul A.
Detection of Cytomegalovirus in Whole Blood Using Three Different Real-Time PCR Chemistries  Erica Vincent, Zhengming Gu, Markus Morgenstern, Candace.
Laboratory Diagnosis of Clostridium difficile Infection
Joanna Wang, Chetan Bettegowda  The Journal of Molecular Diagnostics 
Single Nucleotide Polymorphism-Based System Improves the Applicability of Quantitative PCR for Chimerism Monitoring  Egle Gineikiene, Mindaugas Stoskus,
Evaluation and Clinical Validation of Two Field–Deployable Reverse Transcription- Insulated Isothermal PCR Assays for the Detection of the Middle East.
Plasmodium Detection and Differentiation by Direct-on-Blood PCR Nucleic Acid Lateral Flow Immunoassay  Johanna M. Roth, Laura de Bes, Patrick Sawa, George.
Cornelis J. J. Huijsmans, Jeroen Poodt, Paul H. M. Savelkoul, Mirjam H
Ken B. Waites, Li Xiao, Vanya Paralanov, Rose M. Viscardi, John I
Single-Run, Parallel Detection of DNA from Three Pneumonia-Producing Bacteria by Real-Time Polymerase Chain Reaction  Reinhard B. Raggam, Eva Leitner,
A Lean Laboratory The Journal of Molecular Diagnostics
Implementation of Formalin-Fixed, Paraffin-Embedded Cell Line Pellets as High-Quality Process Controls in Quality Assessment Programs for KRAS Mutation.
Rapid Real-Time PCR Assays for Detection of Klebsiella pneumoniae with the rmpA or magA Genes Associated with the Hypermucoviscosity Phenotype  Laurie.
Patrick R. Murray  The Journal of Molecular Diagnostics 
Development of a Rapid Automated Influenza A, Influenza B, and Respiratory Syncytial Virus A/B Multiplex Real-Time RT-PCR Assay and Its Use during the.
William L. Gerald, M.D., Ph.D, 1954–2008
Detection and Typing of Viruses Using Broadly Sensitive Cocktail-PCR and Mass Spectrometric Cataloging  Charul Gijavanekar, Rafal Drabek, Mithil Soni,
Real-Time Polymerase Chain Reaction for Detecting Bacterial DNA Directly from Blood of Neonates Being Evaluated for Sepsis  Jeanne A. Jordan, Mary Beth.
Impact of Rapid Molecular Respiratory Virus Testing on Real-Time Decision Making in a Pediatric Emergency Department  Daniel T. Rogan, Mohit S. Kochar,
Development and Clinical Implementation of a Combination Deletion PCR and Multiplex Ligation-Dependent Probe Amplification Assay for Detecting Deletions.
Real-Time Polymerase Chain Reaction Detection of Herpes Simplex Virus in Cerebrospinal Fluid and Cost Savings from Earlier Hospital Discharge  Kenneth.
Benjamin P. Song, Surbhi Jain, Selena Y. Lin, Quan Chen, Timothy M
Christine Formisano-Tréziny, Marina de San Feliciano, Jean Gabert 
Detection of human metapneumovirus and respiratory syncytial virus by duplex real-time RT-PCR assay in comparison with direct fluorescent assay  P. Jokela,
Comparative Evaluation of Four Real-Time PCR Methods for the Quantitative Detection of Epstein-Barr Virus from Whole Blood Specimens  Daelynn Buelow,
BRAF Mutation Testing in Solid Tumors
Comparative Evaluation of Broad-Panel PCR Assays for the Detection of Gastrointestinal Pathogens in Pediatric Oncology Patients  Zhengming Gu, Haiqing.
Development of a Quantitative Real-Time Polymerase Chain Reaction Assay for the Detection of the JAK2 V617F Mutation  Elizabeth C. Wolstencroft, Katy.
Multiplex Bead Suspension Array for Screening Neisseria gonorrhoeae Antibiotic Resistance Genetic Determinants in Noncultured Clinical Samples  Sergey.
The Molecular Pathology of Primary Immunodeficiencies
Modified Real-Time PCR for Detecting, Differentiating, and Quantifying Ureaplasma urealyticum and Ureaplasma parvum  Ellen Vancutsem, Oriane Soetens,
Highly Sensitive Droplet Digital PCR Method for Detection of EGFR-Activating Mutations in Plasma Cell–Free DNA from Patients with Advanced Non–Small Cell.
Application of Nuclear Magnetic Resonance to Detect Toxigenic Clostridium difficile from Stool Specimens  Paul Yang, Sara Hash, Katherine Park, Charlene.
Design and Evaluation of a Real-Time PCR Assay for Quantification of JAK2 V617F and Wild-Type JAK2 Transcript Levels in the Clinical Laboratory  Jason.
Epidemiology of viral respiratory infections in a tertiary care centre in the era of molecular diagnosis, Geneva, Switzerland, 2011–2012  J. Ambrosioni,
Evaluating the Effect of Unclassified Variants Identified in MMR Genes Using Phenotypic Features, Bioinformatics Prediction, and RNA Assays  Lucia Pérez-Cabornero,
Retrospective Comparison of Nucleic Acid Sequence–Based Amplification, Real-Time PCR, and Galactomannan Test for Diagnosis of Invasive Aspergillosis 
Next-Generation Sequencing for Infectious Disease Diagnosis and Management  Martina I. Lefterova, Carlos J. Suarez, Niaz Banaei, Benjamin A. Pinsky  The.
Rapid and Sensitive Real-Time Polymerase Chain Reaction Method for Detection and Quantification of 3243A>G Mitochondrial Point Mutation  Rinki Singh,
R. Slinger, I. Moldovan, N. Barrowman, F. Chan 
A Sample Extraction Method for Faster, More Sensitive PCR-Based Detection of Pathogens in Blood Culture  John F. Regan, Manohar R. Furtado, Maxim G. Brevnov,
Multiplex PCR Detection of GSTM1, GSTT1, and GSTP1 Gene Variants
A Novel Method to Compensate for Different Amplification Efficiencies between Patient DNA Samples in Quantitative Real-Time PCR  Jules Meijerink, Caroline.
Optimized Allele-Specific Real-Time PCR Assays for the Detection of Common Mutations in KRAS and BRAF  Alois H. Lang, Heinz Drexel, Simone Geller-Rhomberg,
Use of Pyrosequencing of 16S rRNA Fragments to Differentiate between Bacteria Responsible for Neonatal Sepsis  Jeanne A. Jordan, Allyson R. Butchko, Mary.
Two Novel Methods for Rapid Detection and Quantification of DNMT3A R882 Mutations in Acute Myeloid Leukemia  Melissa Mancini, Syed Khizer Hasan, Tiziana.
Karen Snow-Bailey, Ph.D., 1961–2006
Development and Comparison of a Rapid Isothermal Nucleic Acid Amplification Test for Typing of Herpes Simplex Virus Types 1 and 2 on a Portable Fluorescence.
Custom Design of a GeXP Multiplexed Assay Used to Assess Expression Profiles of Inflammatory Gene Targets in Normal Colon, Polyp, and Tumor Tissue  Janice.
Journal of Investigative Dermatology
Improved Detection of the KIT D816V Mutation in Patients with Systemic Mastocytosis Using a Quantitative and Highly Sensitive Real-Time qPCR Assay  Thomas.
Clinical Experience with the Cervista HPV HR Assay
Angiotensin I-Converting Enzyme
Monique A. Johnson, Marvin J. Yoshitomi, C. Sue Richards 
Optimized Allele-Specific Real-Time PCR Assays for the Detection of Common Mutations in KRAS and BRAF  Alois H. Lang, Heinz Drexel, Simone Geller-Rhomberg,
Real-Time Polymerase Chain Reaction Detection of Herpes Simplex Virus in Cerebrospinal Fluid and Cost Savings from Earlier Hospital Discharge  Kenneth.
Presentation transcript:

Development and Laboratory Evaluation of a Real-Time PCR Assay for Detecting Viruses and Bacteria of Relevance for Community-Acquired Pneumonia  Alicia Edin, Susanne Granholm, Satu Koskiniemi, Annika Allard, Anders Sjöstedt, Anders Johansson  The Journal of Molecular Diagnostics  Volume 17, Issue 3, Pages 315-324 (May 2015) DOI: 10.1016/j.jmoldx.2015.01.005 Copyright © 2015 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

Figure 1 Number of quantitative real-time PCR cycles between clinical specimens and positive control spikes with the same amount of target nucleic acid to reveal possible amplification inhibition. PIV, parainfluenza virus; RSV, respiratory syncytial virus. The Journal of Molecular Diagnostics 2015 17, 315-324DOI: (10.1016/j.jmoldx.2015.01.005) Copyright © 2015 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions