TSA dose-dependent increase in transcription.

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TSA dose-dependent increase in transcription. TSA dose-dependent increase in transcription. (A) RNA FISH showing the intensity of single CCND1-MS2 sites of transcription (arrows, cells in G1) in control and TSA-treated cells (7 h). Scale bar, 5 μm. (B) Real-time RT–PCR (qRT–PCR) plots showing the changes in CCND1-MS2 mRNA levels in control and TSA-treated cells, under the regulation of the CMV and CCND1 promoters. Levels are relative to the control. Tubulin and 18S rRNA served as loading controls. Mean ± SD are plotted. (C) Plot showing single molecule RNA FISH quantification of the number of nascent CCND1-MS2 mRNAs on the duplicated active sites of transcription in control and TSA-treated cells (7 h). (D) Fold-induction levels of transcriptional activity of single genes after TSA treatment compared with untreated cells (designated as 1). Number of alleles quantified (n) is marked in red. Sharon Yunger et al. LSA 2018;1:e201800086 © 2018 Yunger et al.