Volume 64, Issue 1, Pages 2-10 (July 2003)

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Volume 64, Issue 1, Pages 2-10 (July 2003) Pathogenesis of nephrogenic diabetes insipidus by aquaporin-2 C-terminus mutations Tomoki Asai, Michio Kuwahara, Hidetake Kurihara, Tatsuo Sakai, Yoshio Terada, Fumiaki Marumo, Sei Sasaki Kidney International Volume 64, Issue 1, Pages 2-10 (July 2003) DOI: 10.1046/j.1523-1755.2003.00049.x Copyright © 2003 International Society of Nephrology Terms and Conditions

Figure 1 Immunoblot of membrane fractions of the Madin-Darby canine kidney (MDCK) cells transfected with the wild-type aquaporin-2 (AQP2) (A), mutant AQP2 (B), and both wild-type and mutant AQP2s (C). The protein expression was detected with an affinity-purified antibody against the wild-type (A), mutant AQP2 (B), or a mixture of both (C). Kidney International 2003 64, 2-10DOI: (10.1046/j.1523-1755.2003.00049.x) Copyright © 2003 International Society of Nephrology Terms and Conditions

Figure 2 Immunocytochemical localization of the wild-type aquaporin-2 (AQP2) in Madin-Darby canine kidney (MDCK) cells transfected with the wild-type AQP2 and mock-transfected MDCK cells. The samples were viewed with a confocal microscope at ×400. Localization of the wild-type AQP2 (A), ZO-1 (B), and merged (C). Localization of the wild-type AQP2 and Na-K-ATPase before (D) and after forskolin treatment (E). Mock-transfected cells (F) stained with antibody against the wild-type AQP2. XY and XZ sections are shown in the upper and lower panels, respectively. White bars indicate 10 μm. Kidney International 2003 64, 2-10DOI: (10.1046/j.1523-1755.2003.00049.x) Copyright © 2003 International Society of Nephrology Terms and Conditions

Figure 3 Immunocytochemical localization of the mutant aquaporin-2 (AQP2) in Madin-Darby canine kidney (MDCK) cells transfected with the mutant AQP2 and mock-transfected MDCK cells. The samples were viewed at ×400X. Localization of the mutant AQP2 (A), Na-K-ATPase (B), merged with (C) or without forskolin treatment (D). In contrast to (A to C), the red and green fluorescence represent the staining of the mutant AQP2 and Na-K-ATPase, respectively, in (D). Mock-transfected cells stained with antibody against the mutant AQP2 (E). Determination of the cross-reactivity of antibodies. Cells were incubated with rabbit antimutant AQP2 antibody, and then incubated with antibodies against rabbit IgG (F) or mouse IgG (G). XY and XZ sections are shown in the upper and lower panels, respectively. White bars indicate 10 μm. Kidney International 2003 64, 2-10DOI: (10.1046/j.1523-1755.2003.00049.x) Copyright © 2003 International Society of Nephrology Terms and Conditions

Figure 4 Immunocytochemical localization of the wild-type and mutant aquaporin-2 (AQP2) in cotransfected Madin-Darby canine kidney (MDCK) cells. The wild-type and mutant AQP2s were stably cotransfected in MDCK cell. Colocalization of the wild-type AQP2 (A), Na-K-ATPase (B), and merged (C) was observed at ×400. Colocalization of the wild-type AQP2s (D), the mutant AQP2 (E), and merged (F) merged. Determination of the cross-reactivity of antibodies. Cells were incubated with rabbit anti-wild-type AQP2 antibody, and then incubated with antibodies against rabbit IgG (H) or mouse IgG (I). In addition, cells were incubated with rat anti-wild-type AQP2 antibody, and then incubated with antibodies against rat IgG (J) or rabbit IgG (K). XY and XZ sections are shown in the upper and lower panels, respectively. White bars indicate 10 μm. Kidney International 2003 64, 2-10DOI: (10.1046/j.1523-1755.2003.00049.x) Copyright © 2003 International Society of Nephrology Terms and Conditions

Figure 5 Immunoprecipitation of the wild-type and mutant aquaporin-2 (AQP2) in cotransfected Madin-Darby canine kidney (MDCK) cells. (A) Samples were immunoprecipitated with a wild-type (WT) AQP2 antibody and immunoblotted with the same antibody (upper panel) or an antibody against mutant (M) AQP2 (lower panel). (B) Samples were immunoprecipitated with an antibody to the mutant AQP2 and immunoblotted with an antibody to the wild-type AQP2 (upper panel) or mutant AQP2 (lower panel). Kidney International 2003 64, 2-10DOI: (10.1046/j.1523-1755.2003.00049.x) Copyright © 2003 International Society of Nephrology Terms and Conditions

Figure 6 Localization of Lamp1 in Madin-Darby canine kidney (MDCK) cells stably cotransfected with the wild-type and mutant aquaporin-2 (AQP2). Double staining of Lamp1 (A), ZO-1 (B), merged (C) or Lamp1 (D), Na-K-ATPase (E), or merged (F). XY and XZ sections are shown in the upper and lower panels, respectively. The samples were viewed at ×400 and white bars indicate 10 μm. Kidney International 2003 64, 2-10DOI: (10.1046/j.1523-1755.2003.00049.x) Copyright © 2003 International Society of Nephrology Terms and Conditions