Control and Prevention of emerging and future pathogens at cellular and molecular level throughout the food chain Prof. Mogens Jakobsen Dept. of Food Science The Royal Veterinary and Agricultural University, Copenhagen, Denmark (mogens.jakobsen@kvl.dk) Coordinator of PathogenCombat

PathogenCombat Objective To provide new essential information and methods to the food industry and public authorities on how to reduce the prevalence of new and re-emerging foodborne pathogens Nyhedsværdien, Videnskabelige/tekniske niveau og koordinering = positiv udfald.

Pathogens Bacteria Gram+: Listeria monocytogenes, Mycobacterium avium subsp. paratuberculosis Gram-: Campylobacter jejuni & shiga-like toxin producing Escherichia coli (STEC) Yeast Invasive variants of Saccharomyces cerevisiae Fungi An ochratoxin A producing filamentous fungus (Penicillium nordicum) Viruses Hepatitis E & tickborne encephalitis virus Heat stable bacterial toxins Staphylococcus aureus

HIGH TECHNOLOGY PLATFORMS Pathogen matrix interactions Bioimaging, Flourescent Ratio Imaging Microscopy (FRIM), Force Scope, Confocal Laser Scanning Microscopy (CLSM), Atomic Force Microscopy (AFM) Nyhedsværdien, Videnskabelige/tekniske niveau og koordinering = positiv udfald.

HIGH TECHNOLOGY PLATFORMS Host-pathogen interaction Nyhedsværdien, Videnskabelige/tekniske niveau og koordinering = positiv udfald. Mamalian functional cell models to replace experimental animals

HIGH TECHNOLOGY PLATFORMS Detection and monitoring Functional genomic and bioinformatic based development of DNA-microarrays for pathogen detection viability/stress response/virulence Nyhedsværdien, Videnskabelige/tekniske niveau og koordinering = positiv udfald.

HIGH TECHNOLOGY PLATFORMS Robotic system High Throughput Screening (HTS) for thousands of protective and probiotic cultures Nyhedsværdien, Videnskabelige/tekniske niveau og koordinering = positiv udfald.

HIGH TECHNOLOGY PLATFORMS Modelling microbial population behaviour throughout the food chain Identification of CCP by use of stochastic simulation models based upon quantification of numbers of pathogens including mycotoxin producing fungi and their virulence/toxcicity expression along the entire food chain Estimation of microbial food safety risk at time of consumption based upon stochastic simulation modelling of number of pathogens and their virulence/toxcicity expression Nyhedsværdien, Videnskabelige/tekniske niveau og koordinering = positiv udfald.

PathogenCombat Deliverable I + II New approach to estimate the prevalence of virulent pathogens up to the time of consumption New methods to control the viability and virulence of pathogens throughout the food chain until the time of consumption

FRIM

FRIM - Simultaneous detection of physiological status by intracellular pH-determination Listeria monocytogenes

Force scope – quantifying the microbial adhesion by a novel technique

PathogenCombat Deliverable III New method for estimation of host-pathogen interaction based upon functional cell models

Novel processing technologies to inactivate pathogens PathogenCombat Deliverable IV Novel processing technologies to inactivate pathogens New probiotic and protective cultures to eliminate pathogens Elimination of biofilms based upon hygienic design

New mathematical models covering food at time of consumption PathogenCombat Deliverable V - VII Established strategies and actions for disseminating project deliverables for food SMEs New mathematical models covering food at time of consumption New cost effective food safety management systems

PathogenCombat Deliverable VIII Improved awareness of food safety for the European consumer, food industries and regulatory agencies

PathogenCombat 5 year Integrated Project RTD I RTD II RTD III RTD IV Discovery and Development Detection & Virulence traits Control & Prevention Application and Management NTC I SME network NTC II Training activities NTC III Consumer awareness, food industry and regulatory agencies Total budget 14.3 Mio € EU grant to the budget 11,3 Mio €

Training Training of SME food producing partners and implementation of project deliverables Workshop for about 150 European, INCO and Accession Country SMEs and regulatory agencies Pan European dissemination of deliverables via generic food safety management system Exchange of scientists (40 + 10 INCO/Accession Countries)