Detection of Mycobacterium chelonae, Mycobacterium abscessus Group, and Mycobacterium fortuitum Complex by a Multiplex Real-Time PCR Directly from Clinical.

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Detection of Mycobacterium chelonae, Mycobacterium abscessus Group, and Mycobacterium fortuitum Complex by a Multiplex Real-Time PCR Directly from Clinical Samples Using the BD MAX System Talita T. Rocchetti, Suzane Silbert, Alicia Gostnell, Carly Kubasek, Antonio C. Campos Pignatari, Raymond Widen The Journal of Molecular Diagnostics Volume 19, Issue 2, Pages 295-302 (March 2017) DOI: 10.1016/j.jmoldx.2016.10.004 Copyright © 2017 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

Figure 1 A: Set of common primer forward (PF) and primer reverse (PR) and specific probe (Pb) CY5 for Mycobacterium chelonae (MC) and JOE for Mycobacterium abscessus group (MAG) (subspecies abscessus, massiliense, and bolletii) designed using internal transcribed spacer sequence (ITS) gene. B: Set of primer forward 1 (PF1) for Mycobacterium porcinum, Mycobacterium houstonense, Mycobacterium senegalense, Mycobacterium farcinogenes, and Mycobacterium boenickei; primer forward 2 (PF2) for Mycobacterium fortuitum, Mycobacterium peregrinum, Mycobacterium neworleansense, Mycobacterium septicum, and Mycobacterium alvei; primer reverse (PR) and specific probe (Pb) for M. fortuitum complex (MFC) designed using β subunit of bacterial RNA polymerase (rpoB) gene. The Journal of Molecular Diagnostics 2017 19, 295-302DOI: (10.1016/j.jmoldx.2016.10.004) Copyright © 2017 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions