Spectroscopy and Beer’s Law

Slides:

Advertisements

Similar presentations

Introduction to Spectrophotometry & Beer’s Law

Advertisements

Absorbance spectroscopy

Colorimetry & Spectrophotometry.

Absorbance spectroscopy

Experiment 6 Amount of Dye in a Sports Drink. Goal To make a Beer’s Law standard curve To use the standard curve (and spectrophotometry) to determine.

Introduction to Spectrophotometry

Introduction to Spectrophotometry

Introduction to Spectrophotometry. Why Spectrophotometry? Imagine you are to make a 1μM solution of a specific protein that you believe could have anti-carcinogenic.

Lecture 2b. Electromagnetic Spectrum Visible range: = nm Ultraviolet: = nm Low energyHigh energy.

Scanning spectrophotometry and spectrophotometric determination of concentration BCH 333 [practical]

Determination of Concentration Using Spectrophotometry

Principles of instrumentation Prepared by: Ibtisam H. AlAswad Reham S. Hammad.

Dilution 2003 Required D. Information Given A student is instructed to determine the concentration of a solution of CoCl 2 based on absorption of light.

Let’s Talk About Beer Beer’s Law and Concentrations.

Colorimetry & Spectrophotometry.

Spectrophotometer Prof.Dr. Moustafa M. Mohamed Vice Dean Faculty of Allied Medical Science Pharos University in Alexandria, EGYPT.

What is Spectroscopy?  There are about 12 types of spectroscopy.  It involves ALL parts of the electromagnetic spectrum  We are specifically studying:

Visible Spectrophotometer

5.3 Notes Light & Spectrometry Pg Theory of Light  Color is a visual indication of the fact that objects absorb certain portions of visible.

Exp. 18: Spectrophotometric Analysis: Concentration of a Solution Using Beer’s Law White light violet (400nm) – red (800nm) “visible spectrum” sample (light.

ENVE 201 Environmental Engineering Chemistry 1 COLORIMETRY Dr. Aslıhan Kerç.

Introduction to Spectrophotometry

Colorimeters or photometers

More Solutions Stuff! Raoult’s Law (volatile)

  Examining how much light is absorbed by a compound’s sample at various wavelengths  Spectrum peaks—  Indicates the wavelengths associated with electrons’

SPECTROPHOTOMETRY. Principle : there is interaction between the light and sample particle, spectrophotometer is employed to measure the amount of light.

QUANTITATIVE ANALYSIS Determining amount of ions present in samples.

Determination of Concentration Using Spectrophotometry

Spectrophotometry Ability of molecules to absorb and transmit light energy is the basis of one of the most widely used procedures for determining the concentration.

How can we use spectroscopy to quantify amount of substance?

Introduction to Spectrophotometry & Beer’s Law

Beer’s Law and Concentrations

Solutions & Dilutions Intro to Spectrophotometry.

Atomic Absorption Spectroscopy.  Qualitative test for metals in solution  Can also be used for coloured lights  This emission of light is called an.

5.3 Notes Light & Spectrometry Pg Objectives   Appreciate the phenomenon of how an atom absorbs and releases energy in the form of light 

Spectroscopy and Atomic Spectra A satellite orbiting the Earth contain gravitational potential energy. The satellite can orbit the Earth at any height.

Lab Tools of Forensic Scientists. Detection Presumptive tests ◦ Give idea as to what probably is in a sample/what definitely is not in a sample  Ex:

Spectroscopy Atomic emission spectroscopy (AES)

Principles of instrumentation. Photometry - Photometry means “the measurement of light” If a substance can be converted to a soluble, colored material,

Introduction to Spectrophotometry

Absorption Spectrums 9/18/15.

Laboratory equipment Lecture (4).

Beer-Lambert law Gihan Gawish.Dr.

Principles and practice of Spectrophotometer

Colorimetry GT Chemistry 5/13/15.

Introduction to Spectrophotometry

Estimation of analyte concentration on the basis of light absorption

Colorimeters or photometers

Beer’s Law P0 Uses of Beer’s Law

Introduction to Spectrophotometry

Principles of Spectrophotometer

Absorbance spectroscopy

Spectroscopy Chem honors.

Colorimetry and Beer’s Law

Spectrophotometry For high school Chemistry, AP Chemistry,

Beer’s Law Colorimetry Colligative Properties Review

Lecture 2b Beer’s Lambert Law.

Beer’s Law and Concentrations

Colorimeters OR photometers

Can observe color using absorption spectroscopy (which looks at the intensity of light absorbed versus intensity of light transmitted)

Lab# 1 Scanning spectrophotometry and spectrophotometric determination of concentration BCH 333 [practical]

Let’s Talk About Beer’s Law

Spectrophotometer.

Beer's- Lambert Law and Standard Curves of concentrations

Cu2+ + 4NH3 → Cu(NH3)42+ (deep blue)

Let’s Talk About Beer’s Law

Sample AP Model Drawing Question

Colourimetry The absorption of light by a coloured solution is directly related to the concentration of the solution. Colourimetry determines the concentration.

Spectrophotometry (A type of Spectroscopy)

Presentation transcript:

Spectroscopy and Beer’s Law Unit 2 Spectroscopy and Beer’s Law

This is a spectrophotometer. Spec-20D+

Spectrophotometers work similarly to this device. Vernier Colorimeter

The spectrophotometer is meant to measure the absorbance of light in a sample.

What is absorbance? A measure of how much light (or electromagnetic radiation) does NOT go through the sample. This means the sample is typically colored.

In the case of the Spec-20D+, or a colorimeter, the portion of the electromagnetic spectrum must be…

the visible light wavelengths. 400 nm 700 nm the visible light wavelengths.

So, what is Beer’s Law? a measurement of the amount of absorption at the wavelength of greatest absorption can determine the concentration of the absorbing species present A = d c A = Absorbance = characteristic absorptivity of the strength of the species d = thickness of the sample c = concentration Create a single number that is constant, k.

So, what is Beer’s Law? A = k c This shows that absorbance (A) is directly proportional to concentration (c). This relationship will allow you to measure the absorbance of known concentrations and plot them. You will then measure the absorbance of an unknown concentration. Your graph will allow to interpolate that absorbance to determine the sample’s concentration. Absorbance Concentration