Allergen microarray detects high prevalence of asymptomatic IgE sensitizations to tropical pollen-derived carbohydrates Clarissa R. Cabauatan, PhD, Christian.

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Presentation transcript:

Allergen microarray detects high prevalence of asymptomatic IgE sensitizations to tropical pollen-derived carbohydrates Clarissa R. Cabauatan, PhD, Christian Lupinek, MD, Sandra Scheiblhofer, PhD, Richard Weiss, PhD, Margarete Focke-Tejkl, PhD, Prem L. Bhalla, PhD, Mohan B. Singh, PhD, Paul A. Knight, MSc, Marianne van Hage, MD, PhD, John Donnie A. Ramos, PhD, Rudolf Valenta, MD Journal of Allergy and Clinical Immunology Volume 133, Issue 3, Pages 910-914.e5 (March 2014) DOI: 10.1016/j.jaci.2013.10.004 Copyright © 2013 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 1 IgE reactivities of symptomatic and asymptomatic individuals from the Philippines to micro-arrayed allergens. A, IgE reactivities (y-axes: ISU) of symptomatic (dots) and asymptomatic individuals (triangles) to allergens on the chip (x-axes: allergens, allergen sources, number of positive sera, allergens with known glycosylation are underlined). B, Summary of the frequencies of IgE reactivities in symptomatic and asymptomatic individuals according to allergen sources. Journal of Allergy and Clinical Immunology 2014 133, 910-914.e5DOI: (10.1016/j.jaci.2013.10.004) Copyright © 2013 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 2 A, IgE reactivity (y-axis) of sera from subjects with grass pollen–specific IgE to nCyn d 1 (left panel) and nPhl p 4 (right panel) after preincubation with (+) or without (−) nPhl p 4. B, IgE reactivity (y-axis) of sera to untreated (−) and deglycosylated (+) nCyn d 1 and nPhl p 4. C, Mediator release induced by grass pollen allergens (x-axis) displayed as percentages of total β-hexosaminidase (y-axes) for symptomatic and asymptomatic subjects or buffer. Journal of Allergy and Clinical Immunology 2014 133, 910-914.e5DOI: (10.1016/j.jaci.2013.10.004) Copyright © 2013 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E1 Presence of allergens in timothy grass and tropical grasses. Pollen extracts were subjected to SDS-PAGE and silver stained (lanes a), and after Western blotting, analyzed by using rabbit antisera specific for grass pollen allergen groups (lanes α group 1, 2, 5, 6, 7, and 13), normal rabbit antibodies (lanes nra), or buffer (lanes b). Molecular weights are indicated on the left margins. Journal of Allergy and Clinical Immunology 2014 133, 910-914.e5DOI: (10.1016/j.jaci.2013.10.004) Copyright © 2013 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E2 IgE reactivity of individual sera (y-axis: ISU) to allergens on ImmunoCAP ISAC after periodate treatment (deglycosylated ISAC) or after exposure to buffer alone (untreated ISAC). Journal of Allergy and Clinical Immunology 2014 133, 910-914.e5DOI: (10.1016/j.jaci.2013.10.004) Copyright © 2013 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E3 Quantitative IgE binding (y-axis: kUA/L) of 11 nPhl p 4-positive symptomatic, 3 asymptomatic subjects, and 4 α-Gal–positive sera (positive controls) (x-axis) to α-Gal. Journal of Allergy and Clinical Immunology 2014 133, 910-914.e5DOI: (10.1016/j.jaci.2013.10.004) Copyright © 2013 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E4 Induction of rat basophil leukemia cell degranulation (y-axis) with different concentrations of timothy grass pollen allergens (x-axis) when cells were loaded with serum IgE from a patient allergic to timothy grass pollen versus lack of degranulation when serum from a nonallergic individuals or buffer was used. Journal of Allergy and Clinical Immunology 2014 133, 910-914.e5DOI: (10.1016/j.jaci.2013.10.004) Copyright © 2013 American Academy of Allergy, Asthma & Immunology Terms and Conditions