A Sensitive Gas Chromatography-Mass Spectrometry Assay Reveals Increased Levels of Monohydroxyeicosatetraenoic Acid Isomers in Human Plasma After Extracorporeal Photoimmunotherapy and Under In Vitro Ultraviolet A Exposure  Marisela Bohne, Harald Gollnick  Journal of Investigative Dermatology  Volume 115, Issue 3, Pages 499-503 (September 2000) DOI: 10.1046/j.1523-1747.2000.00053.x Copyright © 2000 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 Gas chromatogram showing the typical pattern of hydroxyeicosanoic acids in human plasma. Conditions of GC-MS analysis: negative ion chemical ionization-selected ion monitoring at m/z 357 (12-HHT as internal standard) and 399 (HETE). Journal of Investigative Dermatology 2000 115, 499-503DOI: (10.1046/j.1523-1747.2000.00053.x) Copyright © 2000 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 2 Hydroxyeicosanoic acid levels after exposure of the "plasma fraction" to increasing UVA doses in the presence of 8-MOP (400 ng per ml). Human plasma containing leukocytes (so-called buffy coat) was diluted to meet ECPI conditions (30 ml plasma + 13.5 ml phosphate-buffered saline), and exposed to UVA irradiation. Zero value without 8-MOP. Values are mean ± SD of three separate determinations in the plasma fraction of one patient. Journal of Investigative Dermatology 2000 115, 499-503DOI: (10.1046/j.1523-1747.2000.00053.x) Copyright © 2000 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 3 Hydroxyeicosanoic acid levels after exposure of the "plasma fraction" to a UVA dose of 2 J per cm2 in the presence of increasing concentrations of 8-MOP. Control: without 8-MOP and UVA radiation. Values are mean ± SD of three separate determinations in the plasma fraction of one patient. Journal of Investigative Dermatology 2000 115, 499-503DOI: (10.1046/j.1523-1747.2000.00053.x) Copyright © 2000 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 4 Enantiomer composition of 5-HETE. Sample preparation and analytical procedures are described in Materials and Methods. Enantiomers were assigned by coinjection with authentic standards. A partial chromatogram is shown. Journal of Investigative Dermatology 2000 115, 499-503DOI: (10.1046/j.1523-1747.2000.00053.x) Copyright © 2000 The Society for Investigative Dermatology, Inc Terms and Conditions