Ciprofloxacin-resistant, CTX-M-15-producing Escherichia coli ST131 clone in extraintestinal infections in Italy M. Cerquetti, M. Giufrè, A. García-Fernández,

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Ciprofloxacin-resistant, CTX-M-15-producing Escherichia coli ST131 clone in extraintestinal infections in Italy M. Cerquetti, M. Giufrè, A. García-Fernández, M. Accogli, D. Fortini, I. Luzzi, A. Carattoli Clinical Microbiology and Infection Volume 16, Issue 10, Pages 1555-1558 (October 2010) DOI: 10.1111/j.1469-0691.2010.03162.x Copyright © 2010 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 1 Genetic relatedness among 18 extended-spectrum ß-lactamase (ESBLs)-producing and/or qnrB1- and aac(6’)-Ib-cr-positive E. coli isolates. Cluster analysis of the pulsed field gel electrophoresis (PFGE) patterns, phylogenetic groups and multilocus sequence typing genotyping, together with the main characteristics of the resistance genotypes are shown. DNA fragments were analysed with the Diversity Database Fingerprinting Software, version 2 (Bio-Rad Laboratories, Hercules, CA, USA). The band tolerance used was 1.0%. Similarity analysis of the PFGE patterns was performed with Dice’s coefficient and clustering by the unweighted pair group method with arithmetic mean. Strains with a coefficient of similarity value ≥80% were considered to belong to the same PFGE clonal group (clusters A and B in the boxes). *blaCTX-M-15 gene integrated in the chromosome. PBRT, polymerase chain reaction-based replicon typing. Clinical Microbiology and Infection 2010 16, 1555-1558DOI: (10.1111/j.1469-0691.2010.03162.x) Copyright © 2010 European Society of Clinical Infectious Diseases Terms and Conditions