Modification of the Beckman-Coulter second-generation enzyme-linked immunosorbent assay protocol improves the reliability of serum antimüllerian hormone.

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Modification of the Beckman-Coulter second-generation enzyme-linked immunosorbent assay protocol improves the reliability of serum antimüllerian hormone measurement  Laurentiu Craciunas, Dr.-Med., Stephen A. Roberts, Ph.D., Allen P. Yates, Ph.D., Alexander Smith, Ph.D., Cheryl Fitzgerald, M.D., Philip W. Pemberton, M.Sc.  Fertility and Sterility  Volume 103, Issue 2, Pages 554-559.e1 (February 2015) DOI: 10.1016/j.fertnstert.2014.10.052 Copyright © 2015 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Modified second-generation (Gen IIm) antimüllerian hormone (AMH) measurement versus original second-generation (Gen II) assay. The thick line shows the best fit with a simple conversion factor (1.51, 95% confidence interval 1.44–1.58). The thin lines show equivalence (solid line) and a conversion factor of 2 (dashed line). Fertility and Sterility 2015 103, 554-559.e1DOI: (10.1016/j.fertnstert.2014.10.052) Copyright © 2015 American Society for Reproductive Medicine Terms and Conditions

Figure 2 Gen I, Gen II, and Gen IIm AMH versus age. Plots of antimüllerian hormone (AMH) concentration (pmol/L) against age (years) for the first-generation Diagnostic Systems Laboratories (Gen I) assay, the original (Gen II) and the modified (Gen IIm) second-generation assays, with the use of linear regression with a quadratic age function. Fertility and Sterility 2015 103, 554-559.e1DOI: (10.1016/j.fertnstert.2014.10.052) Copyright © 2015 American Society for Reproductive Medicine Terms and Conditions

Supplemental Figure 1 Modified second-generation (Gen IIm) antimüllerian hormone (AMH) measurement versus antral follicle count (AFC). The line is a linear fit: log(AMH) = −1.41 + 1.59 log(AFC + 0.5). Fertility and Sterility 2015 103, 554-559.e1DOI: (10.1016/j.fertnstert.2014.10.052) Copyright © 2015 American Society for Reproductive Medicine Terms and Conditions