Zygomycosis: conventional laboratory diagnosis C. Lass-Flörl  Clinical Microbiology and Infection  Volume 15, Pages 60-65 (January 2009) DOI: 10.1111/j.1469-0691.2009.02999.x Copyright © 2009 European Society of Clinical Microbiology and Infectious Diseases Terms and Conditions

FIG. 1 Zygomycosis: diagnostic approach using microscopic and culture techniques. BAL, Broncho-alveolar lavage; TS, Tracheal secretions. Clinical Microbiology and Infection 2009 15, 60-65DOI: (10.1111/j.1469-0691.2009.02999.x) Copyright © 2009 European Society of Clinical Microbiology and Infectious Diseases Terms and Conditions

FIG. 2 Direct microscopic examination. Computed tomography-guided percutaneous lung biopsy stained with Fungi-Fluor shows typically distorted, non-septate and broad hyphae. Clinical Microbiology and Infection 2009 15, 60-65DOI: (10.1111/j.1469-0691.2009.02999.x) Copyright © 2009 European Society of Clinical Microbiology and Infectious Diseases Terms and Conditions

FIG. 3 Periodic acid-Schiff staining of computed tomography-guided percutaneous lung biopsy shows broad-based, ribbon-like, non-septate hyphae with right-angle branching and swollen cells. Clinical Microbiology and Infection 2009 15, 60-65DOI: (10.1111/j.1469-0691.2009.02999.x) Copyright © 2009 European Society of Clinical Microbiology and Infectious Diseases Terms and Conditions

FIG. 4 Microscopic feature of Mucor sp. in culture. Lactophenol cotton blue staining shows a globose intact sporangium with sporangiospores and branched sporangiophores. Stolons and rhizoids are absent. Clinical Microbiology and Infection 2009 15, 60-65DOI: (10.1111/j.1469-0691.2009.02999.x) Copyright © 2009 European Society of Clinical Microbiology and Infectious Diseases Terms and Conditions