Ultrasensitive Detection of KRAS2 Mutations in Bile and Serum from Patients with Biliary Tract Carcinoma Using LigAmp Technology Chanjuan Shi, Arjun.

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Ultrasensitive Detection of KRAS2 Mutations in Bile and Serum from Patients with Biliary Tract Carcinoma Using LigAmp Technology Chanjuan Shi, Arjun Chandrasekaran, Paul J. Thuluvath, Collins Karikari, Pedram Argani, Michael Goggins, Anirban Maitra, James R. Eshleman The Journal of Molecular Diagnostics Volume 11, Issue 6, Pages 583-589 (November 2009) DOI: 10.2353/jmoldx.2009.090061 Copyright © 2009 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 1 LigAmp quantification of KRAS2 mutations in bile from patients with and without biliary tract carcinoma. A: Percentage of mutant KRAS2 relative to wild-type KRAS2 detected in bile from 16 patients with biliary tract carcinoma. LigAmp analysis of GAT (G12D), GTT (G12V), and AGT (G12S) KRAS2 mutations shown as blue, red, and yellow bars, respectively. The mean data were obtained from four individual experiments. Percent mutant KRAS2 = mutant KRAS2/(mutant KRAS2+wild-type KRAS2). The data are presented as mean ± SEM. B: Percentage of mutant KRAS2 relative to wild-type KRAS2 detected in serum from 28 patients with benign pancreatobiliary diseases. The data are presented as mean ± SEM from four independent experiments. The Journal of Molecular Diagnostics 2009 11, 583-589DOI: (10.2353/jmoldx.2009.090061) Copyright © 2009 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 2 Detection of KRAS2 mutations in serum from 11 patients with biliary tract carcinoma. A: The relative amount of mutant to wild-type KRAS2 DNA detected by LigAmp in serum (n = 11). LigAmp analysis of GAT (G12D), GTT (G12V), and AGT (G12S) KRAS2 mutations shown as blue, red and yellow bars, respectively. The mean data were obtained from four individual experiments. The date is presented as mean ± SEM. B: A representative KRAS2 DNA sequence of a cloned BstN1 refractory PCR product from serum of Bile166. A GGT to GAT mutation at codon 12 is shown in the box. The Journal of Molecular Diagnostics 2009 11, 583-589DOI: (10.2353/jmoldx.2009.090061) Copyright © 2009 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions