Zc3h10 downregulation impairs mitochondrial function and C2C12 differentiation Zc3h10 downregulation impairs mitochondrial function and C2C12 differentiation.

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Zc3h10 downregulation impairs mitochondrial function and C2C12 differentiation Zc3h10 downregulation impairs mitochondrial function and C2C12 differentiation AScheme of the experimental plan followed up for Zc3h10 downregulation with adenoviral vectors in proliferating myoblasts.B, CZc3h10 mRNA (B) and protein expression levels (C) with relative quantification in C2C12 myoblasts expressing scramble or Zc3h10 shRNA. n = 3. Statistical analysis was performed by Student's t‐test; *P < 0.05, ***P < 0.001 vs. scramble.DWestern blot of selected Oxphos subunits in isolated mitochondria of C2C12 myoblasts expressing scramble or Zc3h10 shRNA.EmtDNA content in C2C12 myoblasts. n = 3.F, GZc3h10 mRNA (F) and protein levels (G) in differentiating C2C12 myotubes at different time points in cells expressing scramble or Zc3h10 shRNA. n = 3. Statistical analysis was performed by Student's t‐test. ***P < 0.001 vs. scramble.HmtDNA content in C2C12 myotubes at different time points in cells expressing scramble or Zc3h10 shRNA. n = 3.IGene set enrichment analysis (GSEA) of C2C12 myotubes expressing scramble or Zc3h10 shRNA 48 h after induction of differentiation. n = 5.Data information: n indicates the number of biological replicates. Data are expressed as mean ± SD, and P‐values were calculated from the indicated n independent experiments according to [44]. Matteo Audano et al. EMBO Rep. 2018;19:e45531 © as stated in the article, figure or figure legend