Bayesian Inference for Improved Single Molecule Fluorescence Tracking

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Bayesian Inference for Improved Single Molecule Fluorescence Tracking Ji Won Yoon, Andreas Bruckbauer, William J. Fitzgerald, David Klenerman Biophysical Journal Volume 94, Issue 12, Pages 4932-4947 (June 2008) DOI: 10.1529/biophysj.107.116285 Copyright © 2008 The Biophysical Society Terms and Conditions

Figure 1 Synthetic data with five tracks: (a) the first frame of the synthetic image sequence and (b) the ground truth trajectories. Biophysical Journal 2008 94, 4932-4947DOI: (10.1529/biophysj.107.116285) Copyright © 2008 The Biophysical Society Terms and Conditions

Figure 2 Comparison of trajectories detected by a deterministic method with variable thresholds and by SMC method with different sample size: (a) by a deterministic method with a low threshold 22; (b) by a deterministic approach with a proper threshold 50; (c) by a deterministic approach with a high threshold 90; (d) by SMC method with 100 samples used in the Monte Carlo scheme; (e) by SMC method with 1000 samples used; and (f) by SMC method with 2000 samples used. Biophysical Journal 2008 94, 4932-4947DOI: (10.1529/biophysj.107.116285) Copyright © 2008 The Biophysical Society Terms and Conditions

Figure 3 Comparison of variable length tracks: (a) ground truth, (b) deterministic method, and (c) SMC method. Biophysical Journal 2008 94, 4932-4947DOI: (10.1529/biophysj.107.116285) Copyright © 2008 The Biophysical Society Terms and Conditions

Figure 4 The number of spots detected (dimension, K): (a) comparison of the number of spots by deterministic method with four different threshold with 20, 25, 50, 120, and (b) comparison of the number of spots by deterministic method with a threshold 50 and our SMC algorithm. Biophysical Journal 2008 94, 4932-4947DOI: (10.1529/biophysj.107.116285) Copyright © 2008 The Biophysical Society Terms and Conditions

Figure 5 Images with single blinking signals: (a) trajectories for ground truth; (b) trajectories longer than five steps by a deterministic method; and (c) detected tracks longer than five steps by SMC method. Biophysical Journal 2008 94, 4932-4947DOI: (10.1529/biophysj.107.116285) Copyright © 2008 The Biophysical Society Terms and Conditions

Figure 6 Five spots with decreasing SNR with frame number. Biophysical Journal 2008 94, 4932-4947DOI: (10.1529/biophysj.107.116285) Copyright © 2008 The Biophysical Society Terms and Conditions

Figure 7 Images with decreasing intensities at six different time: 1, 5, 70, 80, 90, and 100 (synthetic data). Biophysical Journal 2008 94, 4932-4947DOI: (10.1529/biophysj.107.116285) Copyright © 2008 The Biophysical Society Terms and Conditions

Figure 8 Comparison of trajectories with decreasing intensities (synthetic data): (a) ground truth, (b) trajectories by a deterministic method, and (c) trajectories by SMC. Biophysical Journal 2008 94, 4932-4947DOI: (10.1529/biophysj.107.116285) Copyright © 2008 The Biophysical Society Terms and Conditions

Figure 9 Comparison with experimental data: (a) trajectories longer than five steps detected by the deterministic method; (b) trajectories longer than five steps detected by the SMC method; (c) trajectories longer than 20 steps detected by the deterministic method; and (d) trajectories longer than 20 detected by SMC method. Biophysical Journal 2008 94, 4932-4947DOI: (10.1529/biophysj.107.116285) Copyright © 2008 The Biophysical Society Terms and Conditions

Figure 10 Comparison with a second set of experimental data: (a) 78th frame from the experimental data. (b and c) Full trajectories found by a deterministic method and by SMC, respectively; and panels d–f compare the tracks by a deterministic method and SMC for the same period of time in the dotted and circled region in panels b and c. Biophysical Journal 2008 94, 4932-4947DOI: (10.1529/biophysj.107.116285) Copyright © 2008 The Biophysical Society Terms and Conditions

Figure 11 (a and b) Trajectories found by a deterministic method and SMC respectively for two close spots. (c and d) The deterministic approach can incorrectly link two different tracks. Biophysical Journal 2008 94, 4932-4947DOI: (10.1529/biophysj.107.116285) Copyright © 2008 The Biophysical Society Terms and Conditions