Volume 54, Issue 5, Pages (November 1998)

Slides:

Advertisements

Similar presentations

From: Hepatitis B Virus Strains with Mutations in the Core Promoter in Patients with Fulminant Hepatitis Ann Intern Med. 1995;122(4): doi: /

Advertisements

Clinical Laboratory Analysis of Immunoglobulin Heavy Chain Variable Region Genes for Chronic Lymphocytic Leukemia Prognosis Philippe Szankasi, David.

Xiang Qian, Long Jin, Elzbieta Kulig, Ricardo V. Lloyd

Quantitative Detection and Differentiation of Human Herpesvirus 6 Subtypes in Bone Marrow Transplant Patients by Using a Single Real-Time Polymerase Chain.

Volume 54, Issue 2, Pages (August 1998)

Volume 64, Issue 1, Pages (July 2003)

PCR Polymerase Chain Reaction

Volume 56, Issue 3, Pages (September 1999)

Volume 123, Issue 2, Pages (August 2002)

Volume 119, Issue 5, Pages (November 2000)

An IS6110-targeting fluorescent amplified fragment length polymorphism alternative to IS6110 restriction fragment length polymorphism analysis for Mycobacterium.

Betaine, Dimethyl Sulfoxide, and 7-Deaza-dGTP, a Powerful Mixture for Amplification of GC-Rich DNA Sequences Marco Musso, Renata Bocciardi, Sara Parodi,

Endothelial PAS Domain Protein 1 Chr2: (hg18) Polymorphism Is Associated With Susceptibility to High Altitude Pulmonary Edema in Han Chinese Ying-zhong.

Mutation of a Nuclear Respiratory Factor 2 Binding Site in the 5′ Untranslated Region of the ADSL Gene in Three Patients with Adenylosuccinate Lyase Deficiency

Angiotensin-converting enzyme gene polymorphism and risk of insulin resistance in PCOS Onder Celik, Elif Yesilada, Seyma Hascalik, Nilufer Celik, Ibrahim.

Volume 63, Issue 1, Pages (January 2003)

Volume 54, Issue 2, Pages (August 1998)

Rat mesangial α-endosulfine

Volume 54, Issue 3, Pages (September 1998)

Investigation of the human stem cell factor KIT ligand gene, KITLG, in women with 46,XX spontaneous premature ovarian failure Emily S. Hui, B.A., Ekemini.

Clinical Laboratory Analysis of Immunoglobulin Heavy Chain Variable Region Genes for Chronic Lymphocytic Leukemia Prognosis Philippe Szankasi, David.

DNA-Based Diagnosis of Xeroderma Pigmentosum Group C by Whole-Genome Scan Using Single-Nucleotide Polymorphism Microarray Ching-Wan Lam, Kitty Kit-Ting.

Analysis of an exon 1 polymorphism of the B2 bradykinin receptor gene and its transcript in normal subjects and patients with C1 inhibitor deficiency

Size Polymorphisms in the Human Ultrahigh Sulfur Hair Keratin-Associated Protein 4, KAP4, Gene Family Naoyuki Kariya, Yutaka Shimomura, Masaaki Ito

Izabella Z.A. Pawluczyk, Samita R. Patel, Kevin P.G. Harris

A Rare Mutation in the Primer Binding Region of the Amelogenin Gene Can Interfere with Gender Identification Bonnie Shadrach, Mairead Commane, Carol.

Chimeric RNA/DNA oligonucleotide-based gene therapy

Supplemental Figure 3 A B C T-DNA 1 2 RGLG1 2329bp 3 T-DNA 1 2 RGLG2

Splice Site and Deletion Mutations in Keratin (KRT1 and KRT10) Genes: Unusual Phenotypic Alterations in Scandinavian Patients with Epidermolytic Hyperkeratosis

Volume 65, Issue 6, Pages (June 2004)

Volume 60, Issue 5, Pages (November 2001)

Volume 59, Issue 5, Pages (May 2001)

A microsatellite polymorphism (tttta)n in the promoter of the CYP11a gene in Chinese women with polycystic ovary syndrome Yong Wang, Ph.D., XiaoKe Wu,

Volume 63, Issue 1, Pages (January 2003)

Renal vascular sclerosis is associated with inherited thrombophilias

Volume 119, Issue 4, Pages (October 2000)

Volume 59, Issue 4, Pages (April 2001)

Amplification Refractory Mutation System, a Highly Sensitive and Simple Polymerase Chain Reaction Assay, for the Detection of JAK2 V617F Mutation in Chronic.

Volume 53, Issue 4, Pages (April 1998)

Genetic Analysis of Male Pattern Baldness and the 5α-Reductase Genes

Izabella Z.A. Pawluczyk, Samita R. Patel, Kevin P.G. Harris

S. Alexiou-Daniel, A. Stylianakis, A. Papoutsi, I. Zorbas, A. Papa, A

Ken-Ichi Egoshi, Koichiro Akakura, Takaomi Kodama, Haruo Ito

Sensitive Detection of Polyalanine Expansions in PHOX2B by Polymerase Chain Reaction Using Bisulfite-Converted DNA Hidekazu Horiuchi, Ayako Sasaki, Motoki.

Volume 56, Issue 5, Pages (November 1999)

Lipid-binding proteins in rat and human kidney

Masahide Yazaki, Sandra A. Farrell, Merrill D. Benson

Volume 65, Issue 4, Pages (April 2004)

Wook Lew Journal of Investigative Dermatology

Brad H. Rovin, Ling Lu, Xiaolan Zhang Kidney International

Novel mutation in the nephrin gene of a Japanese patient with congenital nephrotic syndrome of the Finnish type Kunihiko Aya, Hiroyuki Tanaka, Yoshiki.

Volume 55, Issue 3, Pages (March 1999)

Analytical Evaluation of Primer Engineered Multiplex Polymerase Chain Reaction– Restriction Fragment Length Polymorphism for Detection of Factor V Leiden.

Volume 58, Issue 2, Pages (August 2000)

Volume 63, Issue 4, Pages (April 2003)

Volume 63, Issue 3, Pages (March 2003)

Calcium channel blockers, either amlodipine or mibefradil, ameliorate renal injury in experimental diabetes Guorong Ma, Terri J. Allen, Mark E. Cooper,

Anthony M. Raizis, Martin M. Ferguson, David T. Nicholls, Derek W

Volume 62, Issue 5, Pages (November 2002)

Mutational analysis within the 3′ region of the PKD1 gene

Volume 58, Issue 6, Pages (December 2000)

Volume 63, Issue 6, Pages (June 2003)

Maureen J. O'Sullivan, Peter A. Humphrey, Louis P. Dehner, John D

Keith A. Hansen, M. D. , Yueyi Zhang, M. D. , Robert Colver, M. D

Genotype analysis of the neuropeptide Y (NPY) Y1 and NPY Y5 receptor genes in gonadotropin-releasing hormone–dependent precocious gonadarche Mandi Barker-Gibb,

Possible role of the 4G/5G polymorphism of the plasminogen activator inhibitor 1 gene in the development of asthma Seong H. Cho, MDa, Ian P. Hall, MDb,

Volume 60, Issue 2, Pages (August 2001)

Exon Skipping in IVD RNA Processing in Isovaleric Acidemia Caused by Point Mutations in the Coding Region of the IVD Gene Jerry Vockley, Peter K. Rogan,

Fang Wang, Yunfeng Wang, Jie Ding, Jiyun Yang Kidney International

K. Miura, M. Obama, K. Yun, H. Masuzaki, Y. Ikeda, S. Yoshimura, T

Presentation transcript:

Volume 54, Issue 5, Pages 1659-1669 (November 1998) Polymorphisms of angiotensin converting enzyme and plasminogen activator inhibitor-1 genes in diabetes and macroangiopathy1 Hideki Kimura, Fumitake Gejyo, Yoshiki Suzuki, Satoru Suzuki, Ryoichi Miyazaki, Masaaki Arakawa Kidney International Volume 54, Issue 5, Pages 1659-1669 (November 1998) DOI: 10.1046/j.1523-1755.1998.00139.x Copyright © 1998 International Society of Nephrology Terms and Conditions

Figure 1 DNA sequence of the plasminogen activator inhibitor-1 (PAI-1) promoter. A 200-bp region of PAI-1 gene covering the 4G/5G polymorphic site of the promoter is shown. The forward primer (PAI-1 pr1) and the reverse primer (PAI-1pr2) are underlined. The numbers on the DNA sequence denote the position of the nucleic acids from the start of transcription. The arrow indicates an insertion site of one guanine 675-bp upstream from the start of transcription. Kidney International 1998 54, 1659-1669DOI: (10.1046/j.1523-1755.1998.00139.x) Copyright © 1998 International Society of Nephrology Terms and Conditions

Figure 2 Single-strand conformational polymorphism (SSCP) analysis of polymerase chain reaction (PCR)-amplified DNA. (A) Samples containing single-stranded PAI-1 DNA were separated by electrophoresis on a 10% polyacrylamide gel and stained with a silver reagent. Lanes 2, 8 and 11 are 4G4G homozygote. Lanes 1, 3 to 7, and 10 are 4G5G heterozygote. Lanes 9 and 12 are 5G5G homozygote. (B) Three distinct separation patterns (A, B and C) by SSCP analysis are illustrated. Lanes 2, 8 and 11 show pattern A. Lanes 1, 3 to 7, and 10 show pattern B. Lanes 9 and 12 show pattern C. Kidney International 1998 54, 1659-1669DOI: (10.1046/j.1523-1755.1998.00139.x) Copyright © 1998 International Society of Nephrology Terms and Conditions

Figure 3 DNA sequences of the 4G/5G polymorphic region of the PAI-1 promoter. (A) Direct sequencing of the PCR product shown in lane 8 in Figure 1. The 4-guanine tract was verified in both the sense strand (top) and the antisense strand (bottom). (B) Direct sequencing of the PCR product shown in lane 9 in Figure 1. The 5-guanine tract was verified in both the sense strand (top) and the antisense strand (bottom). Kidney International 1998 54, 1659-1669DOI: (10.1046/j.1523-1755.1998.00139.x) Copyright © 1998 International Society of Nephrology Terms and Conditions

Figure 4 Relationship between the PAI-1 4G/5G genotypes and the renal survival rate in patients with NIDDM. There were no significant differences in the renal survival rate among 4G4G genotype (▬), 4G5G genotype () and 5G5G genotype (). Kidney International 1998 54, 1659-1669DOI: (10.1046/j.1523-1755.1998.00139.x) Copyright © 1998 International Society of Nephrology Terms and Conditions

Figure 5 Relationship between the ACE I/D genotypes and the renal survival rate in patients with NIDDM. There were no significant differences in the renal survival rate among DD genotype (▬), ID genotype () and II genotype (). Kidney International 1998 54, 1659-1669DOI: (10.1046/j.1523-1755.1998.00139.x) Copyright © 1998 International Society of Nephrology Terms and Conditions

Kidney International 1998 54, 1659-1669DOI: (10. 1046/j. 1523-1755 Kidney International 1998 54, 1659-1669DOI: (10.1046/j.1523-1755.1998.00139.x) Copyright © 1998 International Society of Nephrology Terms and Conditions

Kidney International 1998 54, 1659-1669DOI: (10. 1046/j. 1523-1755 Kidney International 1998 54, 1659-1669DOI: (10.1046/j.1523-1755.1998.00139.x) Copyright © 1998 International Society of Nephrology Terms and Conditions

Kidney International 1998 54, 1659-1669DOI: (10. 1046/j. 1523-1755 Kidney International 1998 54, 1659-1669DOI: (10.1046/j.1523-1755.1998.00139.x) Copyright © 1998 International Society of Nephrology Terms and Conditions

Kidney International 1998 54, 1659-1669DOI: (10. 1046/j. 1523-1755 Kidney International 1998 54, 1659-1669DOI: (10.1046/j.1523-1755.1998.00139.x) Copyright © 1998 International Society of Nephrology Terms and Conditions

Kidney International 1998 54, 1659-1669DOI: (10. 1046/j. 1523-1755 Kidney International 1998 54, 1659-1669DOI: (10.1046/j.1523-1755.1998.00139.x) Copyright © 1998 International Society of Nephrology Terms and Conditions