Linearity of the RTx assay.

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Linearity of the RTx assay. Linearity of the RTx assay. (A) The upper graph shows real-time PCR fluorescence data as baseline-adjusted relative fluorescence units (RFU) versus the number of PCR cycles. The lower graph shows Tm analysis of the negative value of the change in RFU over the change in temperature (−dRFU/dT) versus temperature (degrees Celsius). (B) Linear relationship between RTx assay CT values and 3′ UTR target copy numbers/reaction. Eff, efficiency. Mulligan E K et al. J. Clin. Microbiol. 2009;47:2635-2638