Sperm DNA fragmentation levels in testicular sperm samples from azoospermic males as assessed by the sperm chromatin dispersion (SCD) test Marcos Meseguer,

Slides:

Advertisements

Similar presentations

A time to look back: analysis of morphokinetic characteristics of human embryo development Javier Herrero, Ph.D., Alberto Tejera, Ph.D., Carmela Albert,

Advertisements

Human blastocyst morphological quality is significantly improved in embryos classified as fast on day 3 (≥10 cells), bringing into question current embryological.

Demián Glujovsky, M.D., M.Sc., Cynthia Farquhar, M.D., M.P.H.

Analysis of the morphological dynamics of blastocysts after vitrification/warming: defining new predictive variables of implantation Aila Coello, Ph.D.,

Study of nucleation status in the second cell cycle of human embryo and its impact on implantation rate Jesus Aguilar, Ph.D., Irene Rubio, Ph.D., Elkin.

A giant oocyte in a cohort of retrieved oocytes: does it have any effect on the in vitro fertilization cycle outcome? Ronit Machtinger, M.D., Joseph.

Report of results obtained in 2,934 women using donor sperm: donor insemination versus in vitro fertilization according to indication Thamara Viloria,

Ratio between inner cell mass diameter and blastocyst diameter is correlated with successful pregnancy outcomes of single blastocyst transfers Miriam.

Marcos Meseguer, Ph. D. , Rebeca Santiso, Ph. D. , Nicolas Garrido, Ph

Antral follicle count (AFC) can be used in the prediction of ovarian response but cannot predict the oocyte/embryo quality or the in vitro fertilization.

The value of fast blastocoele re-expansion in the selection of a viable thawed blastocyst for transfer Yimin Shu, M.D., Ph.D., Jill Watt, Ph.D., Janice.

Patient and cycle characteristics predicting high pregnancy rates with single-embryo transfer: an analysis of the Society for Assisted Reproductive Technology.

Pharmacological stimulation of sperm motility in frozen and thawed testicular sperm using the dimethylxanthine theophylline Thomas Ebner, Ph.D., Gernot.

Fernando Zegers-Hochschild, M.D. Fertility and Sterility

Composition of single-step media used for human embryo culture

Relationship between pre-embryo pronuclear morphology (zygote score) and standard day 2 or 3 embryo morphology with regard to assisted reproductive technique.

Blastocyst transfer does not cause a sex-ratio imbalance

Marcos Meseguer, Ph. D. , Rebeca Santiso, Ph. D. , Nicolás Garrido, Ph

Can an educational DVD improve the acceptability of elective single embryo transfer? A randomized controlled study Nicole Hope, M.B., B.S., Hon., Luk.

Is frozen embryo transfer cycle associated with a significantly lower incidence of ectopic pregnancy? An analysis of more than 30,000 cycles Bo Huang,

High serum FSH levels in men with nonobstructive azoospermia does not affect success of microdissection testicular sperm extraction Ranjith Ramasamy,

Azoospermia: is sample centrifugation indicated

Time-dependent O2 consumption patterns determined optimal time ranges for selecting viable human embryos Alberto Tejera, Ph.D., Javier Herrero, Ph.D.,

The relative contribution of assisted reproductive technologies and ovulation induction to multiple births in the United States 5 years after the Society.

Live birth of twins derived from zona-free oocytes

Lindsay Kroener, M. D. , Gayane Ambartsumyan, M. D. , Ph. D

New strategy for diagnosing embryo implantation potential by combining proteomics and time-lapse technologies Francisco Dominguez, Ph.D., Marcos Meseguer,

Iris G. Insogna, M. D. , Malinda S. Lee, M. D. , M. B. A. , Rebecca M

Reproductive outcomes of testicular versus ejaculated sperm for intracytoplasmic sperm injection among men with high levels of DNA fragmentation in semen:

Optimizing the number of blastocyst stage embryos to transfer on day 5 or 6 in women 38 years of age and older: a Society for Assisted Reproductive Technology.

The significance of sperm DNA oxidation in embryo development and reproductive outcome in an oocyte donation program: a new model to study a male infertility.

Improvements achieved in an oocyte donation program over a 10-year period: sequential increase in implantation and pregnancy rates and decrease in high-order.

The effect of blastomere biopsy on preimplantation mouse embryo development and global gene expression Francesca E. Duncan, Ph.D., Paula Stein, Ph.D.,

Ramadan Saleh, M. D. , Reda Z. Mahfouz, M. D. , Ashok Agarwal, Ph. D

Automatic time-lapse instrument is superior to single-point morphology observation for selecting viable embryos: retrospective study in oocyte donation

Composition of commercial media used for human embryo culture

Optimizing the culture environment and embryo manipulation to help maintain embryo developmental potential Jason E. Swain, Ph.D., Doug Carrell, Ph.D.,

Application of three-dimensional fluorescence in situ hybridization to human preimplantation genetic diagnosis Li-Ying Yan, Ph.D., Jie Qiao, M.D., Yuan.

Additive effect of factors related to assisted conception on the reduction of maternal serum pregnancy-associated plasma protein A concentrations and.

Type of chromosome abnormality affects embryo morphology dynamics

Morphokinetic analysis and embryonic prediction for blastocyst formation through an integrated time-lapse system Yamileth Motato, Ph.D., María José de.

Obesity reduces uterine receptivity: clinical experience from 9,587 first cycles of ovum donation with normal weight donors José Bellver, M.D., Antonio.

Using the Society for Assisted Reproductive Technology Clinic Outcome System morphological measures to predict live birth after assisted reproductive.

Normal birth after single-embryo transfer in a patient with excessive polypronuclear zygote formation following in vitro fertilization and intracytoplasmic.

Differential pH in embryo culture

Prediction of pregnancy rate by blastocyst morphological score and age, based on 1,488 single frozen-thawed blastocyst transfer cycles Sakae Goto, M.D.,

Should embryos developing to blastocysts on day 7 be cryopreserved and transferred: an analysis of pregnancy and implantation rates George Kovalevsky,

Value of the sperm deoxyribonucleic acid fragmentation level, as measured by the sperm chromatin dispersion test, in the outcome of in vitro fertilization.

Cumulative newborn rates increase with the total number of transferred embryos according to an analysis of 15,792 ovum donation cycles Nicolás Garrido,

Evaluation of diagnostic testis biopsy and the repetition of testicular sperm extraction surgeries in infertility patients Alayman Hussein, M.B.B.C.H.,

Ana Cobo, Ph. D. , Aila Coello, Ph. D. , Jose Remohí, M. D

Nitric oxide metabolite production in the human preimplantation embryo and successful blastocyst formation Christopher W. Lipari, M.D., Jairo E. Garcia,

Human embryo twinning with applications in reproductive medicine

Storage of human oocytes in the vapor phase of nitrogen

Live babies born per oocyte retrieved in a subpopulation of oocyte donors with repetitive reproductive success J. Ryan Martin, M.D., Jason G. Bromer,

Tong Du, M. S. , Hong Chen, M. D. , Rong Fu, M. S. , Qiuju Chen, Ph. D

José Bellver, M. D. , Yanira Ayllón, M. D. , Marcos Ferrando, M. D

Impact of different patterns of sperm chromosomal abnormalities on the chromosomal constitution of preimplantation embryos Lorena Rodrigo, B.Sc., Vanessa.

Sperm selection by swim-up in terms of deoxyribonucleic acid fragmentation as measured by the sperm chromatin dispersion test is altered in heavy smokers

Sperm deoxyribonucleic acid fragmentation as a prognostic indicator of assisted reproductive technology outcome Mehdi Benchaib, M.D., Ph.D., Jacqueline.

Comparison of microdissection testicular sperm extraction, conventional testicular sperm extraction, and testicular sperm aspiration for nonobstructive.

Yoko Kumasako, B. E. , Eiko Otsu, M. En. , Takafumi Utsunomiya, M. D

Irene Rubio, Ph. D. , Reidun Kuhlmann, Inge Agerholm, Ph. D

Parabolic trend in endometrial thickness at embryo transfer in in vitro fertilization/intracytoplasmic sperm injection cases with clinical pregnancy evidence

In vitro sildenafil citrate use as a sperm motility stimulant

Saad Elzanaty, M.D., Ph.D., Johan Malm, M.D., Ph.D.

Are sperm DNA fragmentation, hyperactivation, and hyaluronan-binding ability predictive for fertilization and embryo development in in vitro fertilization.

Contact among families who share the same sperm donor

Tamer M. Said, M. D. , Geetha Ranga, M. D. , Ashok Agarwal, Ph. D. , H

A time to look back: analysis of morphokinetic characteristics of human embryo development Javier Herrero, Ph.D., Alberto Tejera, Ph.D., Carmela Albert,

Presentation transcript:

Sperm DNA fragmentation levels in testicular sperm samples from azoospermic males as assessed by the sperm chromatin dispersion (SCD) test Marcos Meseguer, Ph.D., Rebeca Santiso, Ph.D., Nicolas Garrido, Ph.D., Manuel Gil-Salom, M.D., Jose Remohí, M.D., Jose Luis Fernandez, M.D. Fertility and Sterility Volume 92, Issue 5, Pages 1638-1645 (November 2009) DOI: 10.1016/j.fertnstert.2008.08.106 Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Graphic representation of the relationship between obstructive and nonobstructive azoospermia, sperm morphology and sperm recovery after thawing (pentoxifylline), and the percentage of testicular sperm with DNA fragmentation. Asterisks (∗) denote a significant difference. Fertility and Sterility 2009 92, 1638-1645DOI: (10.1016/j.fertnstert.2008.08.106) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

Figure 2 Percentage of cells with testicular SDF with respect to classification of embryos on day 3 depending on blastomere symmetry. We defined three types of embryos: type 1, embryo cell volume with equal distribution, which means similar size in even blastomere number embryos, that is, 4, 6, or 8, or one cell with bigger diameter in odd blastomere number embryos; type 3, embryos with one big cell, which is considered dominant and which represents at least 1/3 of the embryo volume; type 2, the rest of the embryos, which includes embryos with odd blastomere number and equal size and embryos with even or odd numbers but different blastomere sizes. Asterisks (∗) denote a significant difference of the percentage of testicular SDF between the types considered (P<.05). A representative picture of each type of classified embryo has been included in the graphic. Fertility and Sterility 2009 92, 1638-1645DOI: (10.1016/j.fertnstert.2008.08.106) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

Figure 3 Classifications of blastocysts as those whose developed blastocoel cavity on day 6 (144 hours after fertilization) compare with the rest of the embryos that did not reach the blastocyst stage, which were considered to be arrested. A comparison of the percentage of cells with testicular SDF with respect to the blastocysts and the arrested embryos was performed. Asterisks (∗) denote a significant difference in the percentage of testicular SDF between both types considered (P<.05). A representative picture of both types of classified embryos has been included in the graphic. Fertility and Sterility 2009 92, 1638-1645DOI: (10.1016/j.fertnstert.2008.08.106) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

Figure 4 Classification of the embryos into three categories depending on the final destination: nonviable, frozen embryo, and transferred embryo. Results from the compilation of these categories with testicular SDF show that increased SDF is associated with nonviable (bad-quality) embryos. Asterisks (∗) denote a significant increase in the percentage of testicular SDF in those nonviable embryos produced compared with frozen and transferred embryos (good quality; P<.05). Fertility and Sterility 2009 92, 1638-1645DOI: (10.1016/j.fertnstert.2008.08.106) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions