Specific depletion of CD4-DTR–derived CD4 T cells.

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Specific depletion of CD4-DTR–derived CD4 T cells. Specific depletion of CD4-DTR–derived CD4 T cells. (A) Lethally irradiated CD4−/− mice were reconstituted with CD4-DTR (CD45.2+) and CD45.1+ B6 BM and persistently infected with 2 × 106 FFU LCMV clone 13. From d20 pi onward, continuous DTx treatment every 3 to 4 days was initiated. (B) Longitudinal analysis of CD4 and CD8 T cell ratios in blood. CD4-DTR–derived CD4/CD8 T cells (CD45.2+) and CD45.1+ B6–derived CD4/CD8 T cells are separately plotted. (C) Ratios and total cell numbers of CD4-DTR–derived CD4 T cells to CD45.1+ B6–derived CD4 T cells in the spleen, mLN, and lung at d40 pi. (D) Viral titers in the spleen on d40 pi. Statistical analysis was performed using Mann-Whitney U test; P = 0.8413. (E) Ratios and numbers of CD4-DTR–derived (CD45.2+) and CD45.1+ B6–derived non-TFH (red gate; CXCR5−) and TFH (blue gate; CXCR5+ PD-1+) cells in the spleen at d40 pi in untreated or DTx-treated animals. Pregated on CD4+ single lymphocytes. One representative experiment of two is shown, n = 4. Error bars represent means ± SD. ns, not significant. Ute Greczmiel et al. Sci. Immunol. 2017;2:eaam8686 Copyright © 2017 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works