Carine Blanchard, PhD, Emily M

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Presentation transcript:

A striking local esophageal cytokine expression profile in eosinophilic esophagitis Carine Blanchard, PhD, Emily M. Stucke, BA, Beatriz Rodriguez-Jimenez, MD, Karen Burwinkel, BS, Margaret H. Collins, MD, Annette Ahrens, BA, Eileen S. Alexander, BS, Bridget K. Buckmeier Butz, MS, Sean C. Jameson, BA, Ajay Kaul, MD, James P. Franciosi, MD, Jonathan P. Kushner, MD, Philip E. Putnam, MD, J. Pablo Abonia, MD, Marc E. Rothenberg, MD, PhD Journal of Allergy and Clinical Immunology Volume 127, Issue 1, Pages 208-217.e7 (January 2011) DOI: 10.1016/j.jaci.2010.10.039 Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 1 Cytokine mRNA levels in patients with EE. Cytokine mRNA levels were quantified by using real-time PCR in 11 healthy subjects and 11 patients with EE. Expression levels were normalized to the housekeeping gene glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and are expressed as a relative ratio. Patients with EE were subdivided on the basis of the maximum eosinophil number in the biopsy into active (>23 eosinophils/hpf), intermediate (partially treated EE with 1-23 eosinophils/hpf), and inactive (0 eosinophils/hpf). P values were calculated by using the Mann-Whitney test: ∗P < .05; ∗∗∗P < .0005. Journal of Allergy and Clinical Immunology 2011 127, 208-217.e7DOI: (10.1016/j.jaci.2010.10.039) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 2 Eotaxin-3, IL5, IL4, IL13, and IL5RA mRNA levels in patients with EE. Levels were quantified using real-time PCR in a large cohort of healthy (NL) subjects and patients with CE and EE. Expression levels were normalized to the housekeeping gene glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and are expressed as a relative ratio. Patients with EE were subdivided on the basis of the maximum eosinophil number in the biopsy into active (>23 eosinophils/hpf), intermediate (Int EE; partially treated EE with 1-23 eosinophils/hpf), and inactive (0 eosinophils/hpf). P values were calculated by using the Kruskal-Wallis test with a Dunn multiple comparison test: ∗P < .05; ∗∗P < .005; ∗∗∗P < .0005. Journal of Allergy and Clinical Immunology 2011 127, 208-217.e7DOI: (10.1016/j.jaci.2010.10.039) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 3 IL4, IL5, IL13, IL5RA, and eotaxin-3 expression in patients with active EE with and without allergy. IL4, IL5, IL13, IL5RA, and eotaxin-3 mRNA levels were quantified using real-time PCR in patients with active EE (>23 eosinophils/hpf) from the large cohort. Expression levels were normalized to the housekeeping gene glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and are expressed as a relative ratio. P values were calculated by using the Mann-Whitney test: ∗∗P < .005; ∗∗∗P < .0005. Journal of Allergy and Clinical Immunology 2011 127, 208-217.e7DOI: (10.1016/j.jaci.2010.10.039) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 4 Correlation and linear regression among IL4, IL5, IL13, and eotaxin-3. IL4, IL5, IL13, and eotaxin-3 mRNA levels were quantified by using real-time PCR in patients with active EE. Spearman correlation r (italics) and P values were calculated to test correlation between the ranked cytokines levels (nonparametric). Linear regression curves are presented with the corresponding P value and r2 (bold) on the curves to test whether the levels of cytokine directly correlate with each other. A nonsignificant P value (NS) indicates that the slope of the curve is not significantly different from 0. Journal of Allergy and Clinical Immunology 2011 127, 208-217.e7DOI: (10.1016/j.jaci.2010.10.039) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 5 Plasma cytokine levels in healthy subjects and patients with EE. A, Blood cytokine levels significantly (P < .05) differentially expressed in healthy subjects and patients with EE are shown for IL-4, IL-5, IL-6, IL-8, IL-17, IL-13, IL-12p70, CD40L, and IL-1α. B, Blood cytokine levels in patients with EE before (active) and after (inactive) therapy. The P values were calculated by using the Mann-Whitney test (A) or paired t test (B). Journal of Allergy and Clinical Immunology 2011 127, 208-217.e7DOI: (10.1016/j.jaci.2010.10.039) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Plasma cytokine levels in healthy subjects and patients with EE Plasma cytokine levels in healthy subjects and patients with EE. Blood cytokine levels are shown as pg/mL. The P values >.05 were calculated by using the Mann-Whitney test. Journal of Allergy and Clinical Immunology 2011 127, 208-217.e7DOI: (10.1016/j.jaci.2010.10.039) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions