Lung-derived mesenchymal stem cells (MSC) display in vitro multilineage potential. Lung-derived mesenchymal stem cells (MSC) display in vitro multilineage.

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Lung-derived mesenchymal stem cells (MSC) display in vitro multilineage potential. Lung-derived mesenchymal stem cells (MSC) display in vitro multilineage potential. MSC derived from central and peripheral transbronchial lung biopsies obtained from six lung-transplanted patients were seeded in an appropriate differentiation-induction medium. Lung-derived MSC differentiated into adipocytes (A), osteoblasts (B) and chondrocytes (C). Adipocytes were stained with Oil-Red-O staining (A and D), osteoblasts with alizarin red (B and E) and chondrocytes with an antiaggrecan antibody (C and F). Control cells were cultured in normal growth medium (D–F). Scale bare for adipocytes, osteoblast and chondrocyte pictures represents 20, 100 and 50 μm, respectively. To confirm in vitro differentiation, we performed reverse transcription-PCR on cultured MSC obtained from three lung-transplanted patients (G–I). Fold change in mRNA expression of peripheral transbronchially derived cells (white) is shown for peroxisome proliferator-activated receptor γ (PPARG; G), alkaline phosphatase (ALPL; H) and aggrecan (ACAN; I) using centrally derived cells (black) as the reference gene. Sara Rolandsson et al. BMJ Open Resp Res 2014;1:e000027 ©2014 by British Thoracic Society