Clinical Microbiology and Infection

Slides:

Advertisements

Similar presentations

Models for the organisation of hospital infection control and prevention programmes B. Gordts Clinical Microbiology and Infection Volume 11, Pages

Advertisements

Candida species distribution and antifungal susceptibility testing according to European Committee on Antimicrobial Susceptibility Testing and new vs.

Azole-resistant Aspergillus fumigatus in Denmark: a laboratory-based study on resistance mechanisms and genotypes R.H. Jensen, F. Hagen, K.M.T. Astvad,

Antimicrobial susceptibility testing of colistin – evaluation of seven commercial MIC products against standard broth microdilution for Escherichia coli,

Evaluation of the in vitro activity of isavuconazole and comparator voriconazole against 2635 contemporary clinical Candida and Aspergillus isolates

R. Dumke, H. von Baum, P.C. Lück, E. Jacobs

Helicobacter pylori resistance to antibiotics in 2014 in France detected by phenotypic and genotypic methods A. Ducournau, L. Bénéjat, E. Sifré, E. Bessède,

L. Boyanova Clinical Microbiology and Infection

Clinical Microbiology and Infection

C.N. van Ettekoven, D. van de Beek, M.C. Brouwer

I.J. Schalk Clinical Microbiology and Infection

I. López-Hernández, M. Delgado Valverde, N. Batista Díaz, A. Pascual

Molecular characterization of methicillin-resistant Staphylococcus aureus isolated over a 2-year period in a Qatari hospital from multinational patients

D. Khalil, M. Hultin, M.U. Rashid, B. Lund

Control of multidrug-resistant Gram-negative bacteria in low- and middle-income countries—high impact interventions without much resources N. Singh,

Migrant health—a cause for concern?

Increased conjugation frequencies in clinical Enterococcus faecium strains harbouring the enterococcal surface protein gene esp B. Lund, H. Billström,

In-vitro susceptibility and molecular characterisation of macrolide resistance mechanisms among Streptococcus pneumonia isolates in The Netherlands: the.

Discontinuation of echinocandin and azole treatments led to the disappearance of an FKS alteration but not azole resistance during clonal Candida glabrata.

Easy synergism for colistin-resistant KPC-producing Klebsiella pneumoniae: the E-test with supplemented agar E. Tagliaferri, C. Tascini, A. Leonildi,

R. Cantón Clinical Microbiology and Infection

Semi-national surveillance of fungaemia in Denmark 2004–2006: increasing incidence of fungaemia and numbers of isolates with reduced azole susceptibility

A.E. Muller, U. Theuretzbacher, J.W. Mouton

Antimicrobial susceptibility of Streptococcus pneumoniae in Latin America: results from five years of the SENTRY Antimicrobial Surveillance Program M.

Azole-resistant Aspergillus fumigatus in Denmark: a laboratory-based study on resistance mechanisms and genotypes R.H. Jensen, F. Hagen, K.M.T. Astvad,

Should standardized susceptibility testing for microbial biofilms be introduced in clinical practice? T. Coenye, D. Goeres, F. Van Bambeke, T. Bjarnsholt

Clinical Microbiology and Infection

Cryptococcus neoformans var

In-vitro activity of nikkomycin Z alone and in combination with polyenes, triazoles or echinocandins against Aspergillus fumigatus L.T. Ganesan, E.K.

H. Adibhesami, M. Douraghi, M. Rahbar, A. Abdollahi

Identification of genetic markers of resistance to echinocandins, azoles and 5- fluorocytosine in Candida glabrata by next-generation sequencing: a feasibility.

Vector control: a cornerstone in the malaria elimination campaign

J.P. Donnelly, B.E. De Pauw Clinical Microbiology and Infection

Candida species distribution and antifungal susceptibility testing according to European Committee on Antimicrobial Susceptibility Testing and new vs.

Method for the determination of minimum inhibitory concentration (MIC) by broth dilution of fermentative yeasts J.L. Rodríguez-Tudela, F. Barchiesi,

E. Vermeulen, J. Maertens, P. Meersseman, V. Saegeman, L. Dupont, K

I. Al Shukri, F. Hamilton, M. Evans, S. Cooper, G. McKenzie, L

Evaluation of vancomycin MIC creep in methicillin-resistant Staphylococcus aureus infections—a systematic review and meta-analysis R. Diaz, V. Afreixo,

Aerococcus: an increasingly acknowledged human pathogen

Sequence-based identification, genotyping and EUCAST antifungal susceptibilities of Trichosporon clinical isolates from Greece M. Arabatzis, P. Abel,

Metagenomics and probiotics

M. Aires-de-Sousa Clinical Microbiology and Infection

Previously unknown species of Aspergillus

J. Meletiadis, K. Leth Mortensen, P. E. Verweij, J. W. Mouton, M. C

J. Garau Clinical Microbiology and Infection

T.M. File Clinical Microbiology and Infection

Systematic review of antibiotic consumption in acute care hospitals

Update on antifungal resistance in Aspergillus and Candida

Harmonised monitoring of antimicrobial resistance in Salmonella and Campylobacter isolates from food animals in the European Union S. Bronzwaer Clinical.

Isolation and in vitro susceptibility to amphotericin B, itraconazole and posaconazole of voriconazole-resistant laboratory isolates of Aspergillus fumigatus

Characterization of clinical strains of Aspergillus terreus complex: molecular identification and antifungal susceptibility to azoles and amphotericin.

T. Schön, P. Miotto, C.U. Köser, M. Viveiros, E. Böttger, E. Cambau

Accurate hepatitis C virus genotyping and selection of optimal therapy: lessons from a St Petersburg strain infection E. Knops, E. Heger Clinical Microbiology.

Current experience in treating invasive zygomycosis with posaconazole

Vaccinations against respiratory tract infections at Hajj

A.P. Underwood, J. Green Clinical Microbiology and Infection

H. Leblebicioglu, C. Eroglu Clinical Microbiology and Infection

An after-hours clinical liaison blood culture service—is it worth it?

Clinical Microbiology and Infection

I. Bitar, A. Piazza Clinical Microbiology and Infection

Changing pattern of candidaemia 2001–2006 and use of antifungal therapy at the University Hospital of Vienna, Austria E. Presterl, F. Daxböck, W. Graninger,

The influence of protein binding on the antibacterial activity of faropenem against Haemophilus influenzae I. Gustafsson, O. Cars Clinical Microbiology.

ESCMID* guideline for the diagnosis and management of Candida diseases 2012: developing European guidelines in clinical microbiology and infectious diseases

Z. Erjavec, H. Kluin-Nelemans, P.E. Verweij

A. van Samkar, M. C. Brouwer, Y. Pannekoek, A. van der Ende, D

Virology: a scientific discipline facing new challenges

J.L. Balcázar Clinical Microbiology and Infection

G.C. Schito Clinical Microbiology and Infection

CMI readers' survey Clinical Microbiology and Infection

The future of diagnostic bacteriology

Presentation transcript:

Clinical Microbiology and Infection How to: EUCAST recommendations on the screening procedure E.Def 10.1 for the detection of azole resistance in Aspergillus fumigatus isolates using four-well azole- containing agar plates J. Guinea, P.E. Verweij, J. Meletiadis, J.W. Mouton, F. Barchiesi, M.C. Arendrup M.C. Arendrup, S. Arikan-Akdagli, F. Barchiesi, M. Castanheira, E. Chryssanthou, N. Friberg, J. Guinea, H. Järv, N. Klimko, O. Kurzai, K. Lagrou, C. Lass-Flörl, M. Mares, T. Matos, J. Meletiadis, C.B. Moore, J.W. Mouton, K. Muehlethaler, T.R. Rogers, C.T. Andersen, A. Velegraki J. Guinea, P.E. Verweij, J. Meletiadis, J.W. Mouton, F. Barchiesi, M.C. Arendrup M.C. Arendrup, S. Arikan-Akdagli, F. Barchiesi, M. Castanheira, E. Chryssanthou, N. Friberg, J. Guinea, H. Järv, N. Klimko, O. Kurzai, K. Lagrou, C. Lass-Flörl, M. Mares, T. Matos, J. Meletiadis, C.B. Moore, J.W. Mouton, K. Muehlethaler, T.R. Rogers, C.T. Andersen, A. Velegraki Clinical Microbiology and Infection DOI: 10.1016/j.cmi.2018.09.008 Copyright © 2018 European Society of Clinical Microbiology and Infectious Diseases Terms and Conditions

Fig. 1 Four-well agar plate with wells filled out with 1 mL of agar media-containing azoles (wells 1–3) or antifungal-free agar (well 4) used as growth control. ITC, itraconazole; VRC, voriconazole; POS, posaconazole. Clinical Microbiology and Infection DOI: (10.1016/j.cmi.2018.09.008) Copyright © 2018 European Society of Clinical Microbiology and Infectious Diseases Terms and Conditions

Fig. 2 Expected growth pattern for the A. fumigatus SSI-4524 QC strain harbouring the TR34/L98H alteration in the CYP51A gene and promoter. Each well of the plate contains the following azoles: well 1 (itraconazole 4 mg/L), well 2 (voriconazole 2 mg/L), well 3 (posaconazole 0.5 mg/L), and well 4 (no azoles, growth control). SSI = Statens Serum Institut, Copenhagen, Denmark. The QC strain is available from the EUCAST Development Laboratory (EDL) for fungi: http://www.eucast.org/organization/developmentlaboratories/. Clinical Microbiology and Infection DOI: (10.1016/j.cmi.2018.09.008) Copyright © 2018 European Society of Clinical Microbiology and Infectious Diseases Terms and Conditions

Fig. 3 Expected growth pattern for the A. fumigatus SSI-5586 QC strain harbouring a G54W substitution in the CYP51A gene. Each well of the plate contains the following azoles: well 1 (itraconazole 4 mg/L), well 2 (voriconazole 2 mg/L), well 3 (posaconazole 0.5 mg/L), and well 4 (no azoles, growth control). SSI, Statens Serum Institut, Copenhagen, Denmark. The QC strain is available from the EUCAST Development Laboratory (EDL) for fungi: http://www.eucast.org/organization/developmentlaboratories/. Clinical Microbiology and Infection DOI: (10.1016/j.cmi.2018.09.008) Copyright © 2018 European Society of Clinical Microbiology and Infectious Diseases Terms and Conditions