Nipple-areolar pigmentation: histology and potential for reconstitution in breast reconstruction  Nicola Dean, Julie Haynes, Jim Brennan, Tim Neild, Chris Goddard, Bronwyn Dearman, Rod Cooter  British Journal of Plastic Surgery  Volume 58, Issue 2, Pages 202-208 (March 2005) DOI: 10.1016/j.bjps.2004.10.027 Copyright © 2004 The British Association of Plastic Surgeons Terms and Conditions

Figure 1 Nipple-areolar reconstruction with a pigmented graft, (A) a few weeks after surgery, and (B) 2 years after surgery. British Journal of Plastic Surgery 2005 58, 202-208DOI: (10.1016/j.bjps.2004.10.027) Copyright © 2004 The British Association of Plastic Surgeons Terms and Conditions

Figure 2 Schematic representation of protocol for tissue sections. British Journal of Plastic Surgery 2005 58, 202-208DOI: (10.1016/j.bjps.2004.10.027) Copyright © 2004 The British Association of Plastic Surgeons Terms and Conditions

Figure 3 Masson–Fontana staining of areolar skin to show melanin (×20). British Journal of Plastic Surgery 2005 58, 202-208DOI: (10.1016/j.bjps.2004.10.027) Copyright © 2004 The British Association of Plastic Surgeons Terms and Conditions

Figure 4 Image analysis of Masson–Fontana stained sections (areolar skin) (×20). British Journal of Plastic Surgery 2005 58, 202-208DOI: (10.1016/j.bjps.2004.10.027) Copyright © 2004 The British Association of Plastic Surgeons Terms and Conditions

Figure 5 Staining of breast skin with Mel-5 antibody, as seen with the image analysis software (melanocytes arrowed) (×20). British Journal of Plastic Surgery 2005 58, 202-208DOI: (10.1016/j.bjps.2004.10.027) Copyright © 2004 The British Association of Plastic Surgeons Terms and Conditions

Figure 6 Growth of melanocytes in medium containing cholera toxin and PMA vs no cholera toxin or PMA. British Journal of Plastic Surgery 2005 58, 202-208DOI: (10.1016/j.bjps.2004.10.027) Copyright © 2004 The British Association of Plastic Surgeons Terms and Conditions